Skip to main content
NCBI home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1971 Oct;50(10):2137–2142. doi: 10.1172/JCI106707

Serum alkaline phosphatase in hypophosphatasia

Joseph B Warshaw 1,2,3,4, John W Littlefield 1,2,3,4, William H Fishman 1,2,3,4, Norma R Inglis 1,2,3,4, Leo L Stolbach 1,2,3,4
PMCID: PMC292147  PMID: 5116205

Abstract

It is recognized that serum alkaline phosphatase may reflect enzyme contributions from bone, liver, and intestine. We have investigated serum alkaline phosphatases in two siblings with hypophosphatasia. After administration of long-chain triglycerides, the major alkaline phosphatase component of their sera was shown to be of intestinal origin on the basis of inhibition by l-phenylalanine. Starch block electrophoresis suggested that there were other regions of l-phenylalanine-sensitive alkaline phosphatase in addition to the major slow-moving intestinal band. Medium-chain triglycerides which are absorbed by the portal route did not cause a similar augmentation of intestinal alkaline phosphatase activity. These studies indicate that serum levels of intestinal alkaline phosphatase are increased normally after long-chain fat feeding in hypophosphatasia and may be the major component of total serum alkaline phosphatase activity.

Full text

PDF
2137

Images in this article

2140Image
on p.2140
2141Image
on p.2141
2141Image
on p.2141

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BECK W. S., VALENTINE W. N. Biochemical studies on leucocytes. II. Phosphatase activity in chronic lymphatic leucemia, acute leucemia, and miscellaneous hematologic conditions. J Lab Clin Med. 1951 Aug;38(2):245–253. [PubMed] [Google Scholar]
  2. BLOMSTRAND R., WERNER B. ALKALINE PHOSPHATASE ACTIVITY IN HUMAN THORACIC DUCT LYMPH. Acta Chir Scand. 1965 Feb;129:177–191. [PubMed] [Google Scholar]
  3. Danovitch S. H., Baer P. N., Laster L. Intestinal alkaline phosphatase activity in familial hypophosphatasia. N Engl J Med. 1968 Jun 6;278(23):1253–1260. doi: 10.1056/NEJM196806062782303. [DOI] [PubMed] [Google Scholar]
  4. FISHMAN W. H., GREEN S., INGLIS N. I. L-phenylalanine: an organ specific, stereospecific inhibitor of human intestinal alkaline phosphatase. Nature. 1963 May 18;198:685–686. doi: 10.1038/198685b0. [DOI] [PubMed] [Google Scholar]
  5. FRASER D. Hypophosphatasia. Am J Med. 1957 May;22(5):730–746. doi: 10.1016/0002-9343(57)90124-9. [DOI] [PubMed] [Google Scholar]
  6. GOYER R. A. Ethanolamine phosphate excretion in a family with hypophosphatasia. Arch Dis Child. 1963 Jun;38:205–207. doi: 10.1136/adc.38.199.205. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Glickman R. M., Alpers D. H., Drummey G. D., Isselbacher K. J. Increased lymph alkaline phosphatase after fat feeding: effects of medium chain triglycerides and inhibition of protein synthesis. Biochim Biophys Acta. 1970 Feb 24;201(2):226–235. doi: 10.1016/0304-4165(70)90296-5. [DOI] [PubMed] [Google Scholar]
  8. HARRIS H., ROBSON E. B. A genetical study of ethanolamine phosphate excretion in hypophosphatasia. Ann Hum Genet. 1959 Dec;23:421–441. doi: 10.1111/j.1469-1809.1959.tb01484.x. [DOI] [PubMed] [Google Scholar]
  9. KRETCHMER N., STONE M., BAUER C. Hereditary enzymatic effects as illustrated by hypophosphatasia. Ann N Y Acad Sci. 1958 Oct 13;75(1):279–285. doi: 10.1111/j.1749-6632.1958.tb36875.x. [DOI] [PubMed] [Google Scholar]
  10. Langman M. J., Constantinopoulos A., Bouchier I. A. ABO blood groups, secretor status, and intestinal mucosal concentrations of alkaline phosphatase. Nature. 1968 Mar 2;217(5131):863–865. doi: 10.1038/217863a0. [DOI] [PubMed] [Google Scholar]
  11. MOSS D. W., KING E. J. Properties of alkaline-phosphatase fractions separated by starch-gel electrophoresis. Biochem J. 1962 Jul;84:192–195. doi: 10.1042/bj0840192. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Newton M. A. A distinctive fraction of alkaline phosphatase in health and disease. J Clin Pathol. 1966 Sep;19(5):491–495. doi: 10.1136/jcp.19.5.491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pimstone B., Eisenberg E., Silverman S. Hypophosphatasia: genetic and dental studies. Ann Intern Med. 1966 Oct;65(4):722–729. doi: 10.7326/0003-4819-65-4-722. [DOI] [PubMed] [Google Scholar]
  14. Posen S. Alkaline phosphatase. Ann Intern Med. 1967 Jul;67(1):183–203. doi: 10.7326/0003-4819-67-1-183. [DOI] [PubMed] [Google Scholar]
  15. SILVERMAN J. L. Apparent dominant inheritance of hypophosphatasia. Arch Intern Med. 1962 Aug;110:191–198. doi: 10.1001/archinte.1962.03620200051010. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES