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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Cryobiology. 2010 Jun 22;61(1):133–141. doi: 10.1016/j.cryobiol.2010.06.007

Figure 9.

Figure 9

The incidence of membrane damage in confluent DPSC monolayers resulting from different freezing profiles performed in the methanol bath. Group 1 – nucleation at −7°C, immediate thawing; Group 2 – nucleation at −7°C, fast two-step cooling; Group 3 – nucleation at −7°C, slow two-step cooling; Group 4 – no nucleation, slow two-step cooling. Unfrozen monolayers acted as the experimental control. Mean ± SEM (n = 8–9). * p < 0.05 vs. untreated control, ** p < 0.05 vs. Group 1 (Mann-Whitney test).