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. 1972 Aug;51(8):1979–1988. doi: 10.1172/JCI107004

Liquid crystalline lipid in the plasma of humans with biliary obstruction

Steven H Quarfordt 1,2,3, Harald Oelschlaeger 1,2,3, William R Krigbaum 1,2,3
PMCID: PMC292354  PMID: 4341011

Abstract

Plasma lipoprotein characteristics were evaluated in a group of patients with obstructed biliary tracts. A 1:1 molar lecithin-free cholesterol liquid crystal phase was observed in the low density flotation region of these patients. The smectic nature of this mesophase was confirmed by electron microscopy, polarized microscopy, and low angle X-ray scattering. A small amount of protein was associated with these liquid crystals, some of which appeared to be components of normal very low and high density lipoproteins. The composition and physical properties of the very low and high density lipoproteins from these obstructed patients differed from normal as well. Aberrant apoprotein patterns were observed for both obstructed very low and high density lipoproteins. A β-electrophoretic migration was observed for a component of these two lipoprotein groups.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ABEL L. L., LEVY B. B., BRODIE B. B., KENDALL F. E. A simplified method for the estimation of total cholesterol in serum and demonstration of its specificity. J Biol Chem. 1952 Mar;195(1):357–366. [PubMed] [Google Scholar]
  2. AHRENS E. H., Jr, KUNKEL H. G. The relationship between serum lipids and skin xanthomata in 18 patients with primary biliary cirrhosis. J Clin Invest. 1949 Nov;28(6 Pt 2):1565–1574. doi: 10.1172/JCI102222. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. AMES B. N., DUBIN D. T. The role of polyamines in the neutralization of bacteriophage deoxyribonucleic acid. J Biol Chem. 1960 Mar;235:769–775. [PubMed] [Google Scholar]
  4. BANGHAM A. D., HORNE R. W. NEGATIVE STAINING OF PHOSPHOLIPIDS AND THEIR STRUCTURAL MODIFICATION BY SURFACE-ACTIVE AGENTS AS OBSERVED IN THE ELECTRON MICROSCOPE. J Mol Biol. 1964 May;8:660–668. doi: 10.1016/s0022-2836(64)80115-7. [DOI] [PubMed] [Google Scholar]
  5. BURSTEIN M., SAMAILLE J. [On a rapid determination of the cholesterol bound to the serum alpha- and beta-lipoproteins]. Clin Chim Acta. 1960 Jul;5:609–609. doi: 10.1016/0009-8981(60)90075-9. [DOI] [PubMed] [Google Scholar]
  6. Bangham A. D., Standish M. M., Watkins J. C. Diffusion of univalent ions across the lamellae of swollen phospholipids. J Mol Biol. 1965 Aug;13(1):238–252. doi: 10.1016/s0022-2836(65)80093-6. [DOI] [PubMed] [Google Scholar]
  7. Brown W. V., Levy R. I., Fredrickson D. S. Studies of the proteins in human plasma very low density lipoproteins. J Biol Chem. 1969 Oct 25;244(20):5687–5694. [PubMed] [Google Scholar]
  8. DE LALLA O. F., GOFMAN J. W. Ultracentrifugal analysis of serum lipoproteins. Methods Biochem Anal. 1954;1:459–478. doi: 10.1002/9780470110171.ch16. [DOI] [PubMed] [Google Scholar]
  9. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  10. FURMAN R. H., CONRAD L. L. Ultracentrifugal characterization of the lipoprotein spectrum in obstructive jaundice: studies of serum lipid relationships in intra-and extrahepatic biliary obstruction. J Clin Invest. 1957 May;36(5):713–722. doi: 10.1172/JCI103474. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Forte T., Norum K. R., Glomset J. A., Nichols A. V. Plasma lipoproteins in familial lecithin: cholesterol acyltransferase deficiency: structure of low and high density lipoproteins as revealed by elctron microscopy. J Clin Invest. 1971 May;50(5):1141–1148. doi: 10.1172/JCI106586. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. GRABAR P. Agar-gel diffusion and immunoelectrophoretic analysis. Ann N Y Acad Sci. 1957 Dec 16;69(4):591–607. doi: 10.1111/j.1749-6632.1957.tb49699.x. [DOI] [PubMed] [Google Scholar]
  13. Hamilton R. L., Havel R. J., Kane J. P., Blaurock A. E., Sata T. Cholestasis: lamellar structure of the abnormal human serum lipoprotein. Science. 1971 Apr 30;172(3982):475–478. doi: 10.1126/science.172.3982.475. [DOI] [PubMed] [Google Scholar]
  14. Howell J. I., Lucy J. A., Pirola R. C., Bouchier I. A. Macromolecular assemblies of lipid in bile. Biochim Biophys Acta. 1970 Jun 9;210(1):1–6. doi: 10.1016/0005-2760(70)90055-x. [DOI] [PubMed] [Google Scholar]
  15. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  16. Lecuyer H., Dervichian D. G. Structure of aqueous mixtures of lecithin and cholesterol. J Mol Biol. 1969 Oct 14;45(1):39–57. doi: 10.1016/0022-2836(69)90208-3. [DOI] [PubMed] [Google Scholar]
  17. Lindgren F. T., Freeman N. K., Ewing A. M., Jensen L. C. Serum lipoprotein distribution, flotation rates and protein analysis. J Am Oil Chem Soc. 1966 May;43(5):281–285. doi: 10.1007/BF02609674. [DOI] [PubMed] [Google Scholar]
  18. Nichols A. V., Gong E. L. Use of sonicated dispersions of mixtures of cholesterol with lecithin as substrates for lecithin:cholesterol acyltransferase. Biochim Biophys Acta. 1971 Feb 2;231(1):175–184. doi: 10.1016/0005-2760(71)90266-9. [DOI] [PubMed] [Google Scholar]
  19. Oncley J. L., Harvie N. R. Lipoproteins--a current perspective of methods and concepts. Proc Natl Acad Sci U S A. 1969 Nov;64(3):1107–1118. doi: 10.1073/pnas.64.3.1107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Papahadjopoulos D., Miller N. Phospholipid model membranes. I. Structural characteristics of hydrated liquid crystals. Biochim Biophys Acta. 1967 Sep 9;135(4):624–638. doi: 10.1016/0005-2736(67)90094-6. [DOI] [PubMed] [Google Scholar]
  21. Picard J., Veissiere D. Abnormal serum lipoprotein in cholestasis: identification and isolation. Clin Chim Acta. 1970 Oct;30(1):149–155. doi: 10.1016/0009-8981(70)90204-4. [DOI] [PubMed] [Google Scholar]
  22. Quarfordt S., Levy R. I., Fredrickson D. S. On thelipoprotein abnormality in type 3 hyperlipoproteinemia. J Clin Invest. 1971 Apr;50(4):754–761. doi: 10.1172/JCI106546. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. RUSS E. M., RAYMUNT J., BARR D. P. Lipoproteins in primary biliary cirrhosis. J Clin Invest. 1956 Feb;35(2):133–144. doi: 10.1172/JCI103257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. SANDBERG D. H., SJOEVALL J., SJOEVALL K., TURNER D. A. MEASUREMENT OF HUMAN SERUM BILE ACIDS BY GAS-LIQUID CHROMATOGRAPHY. J Lipid Res. 1965 Apr;6:182–192. [PubMed] [Google Scholar]
  25. Seidel D., Alaupovic P., Furman R. H., McConathy W. J. A lipoprotein characterizing obstructive jaundice. II. Isolation and partial characterization of the protein moieties of low density lipoproteins. J Clin Invest. 1970 Dec;49(12):2396–2407. doi: 10.1172/JCI106459. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Sweet C., Zull J. E. The binding of serum albumin to phospholipid liposomes. Biochim Biophys Acta. 1970 Dec 1;219(2):253–262. doi: 10.1016/0005-2736(70)90204-x. [DOI] [PubMed] [Google Scholar]
  27. Switzer S. Plasma lipoproteins in liver disease. I. Immunologically distinct low-density lipoproteins in patients with biliary obstruction. J Clin Invest. 1967 Nov;46(11):1855–1866. doi: 10.1172/JCI105676. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. WEINER I. M., GLASSER J. E., LACK L. RENAL EXCRETION OF BILE ACIDS: TAUROCHOLIC, GLYCOCHOLIC, AND COLIC ACIDS. Am J Physiol. 1964 Nov;207:964–970. doi: 10.1152/ajplegacy.1964.207.5.964. [DOI] [PubMed] [Google Scholar]
  29. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]
  30. Woods K. R., Wang K. T. Separation of dansyl-amino acids by polyamide layer chromatography. Biochim Biophys Acta. 1967 Feb 21;133(2):369–370. doi: 10.1016/0005-2795(67)90078-5. [DOI] [PubMed] [Google Scholar]

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