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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Bone. 2010 Jun 25;47(3):657–665. doi: 10.1016/j.bone.2010.06.018

Figure 3.

Figure 3

(A) mRNA levels of ANK, APase, MMP-13, osteocalcin (OC), runx2, type II collagen (α1(II)), and type X collagen α1(X)) of growth plate chondrocytes cultured in the presence of 2.5mM extracellular Pi and in the absence or the presence of RA and PFA. The levels of hypertrophic and terminal differentiation marker and type II collagen mRNAs were determined after 2-day treatment by real-time PCR and SYBR Green and normalized to the 18S RNA levels. Data are means of triplicate PCRs using RNA from three different cultures; error bars represent standard deviations (ap < 0.01 vs. 2.5mM Pi-treated cells; bp < 0.01 vs. 2.5mM Pi/RA-treated cells).