Table 1.
Aggregation midpoint temperatures (TmAgg) of oxidized and reduced Cyt c at different BA concentrations. TmAgg was determined from the peak position in the first derivative spectra of the optical density increase with temperature shown in Figs. 2A & 7A. For reduced Cyt c, complete transition was not observed for 0% v/v and 1% v/v BA, and for 3% v/v BA, a minor transition was observed in addition to the major transition. TmAgg for reduced Cyt c at 1% v/v BA was determined assuming that the increase in optical density reaches the same plateau value as that of 2% v/v and 3% v/v BA. The error bar on the TmAgg value was estimated from the three curves shown for each BA concentration.
| Protein | [BA] (v/v) | TmAgg (°C) |
|---|---|---|
| Cyt c Oxidized |
0 % | 81.4 ± 0.1 |
| 1 % | 75.4 ± 0.1 | |
| 2 % | 67.3 ± 0.1 | |
| 3 % | 57.3 ± 0.1 | |
| Cyt c Reduced |
0 % | > 95 |
| 1 % | 94.8 ± 0.2 | |
| 2 % | 87.5 ± 0.1 | |
| 3 % | 69.0 ± 0.4 78.3 ± 0.1 |