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. 2003 Dec 13;327(7428):1405. doi: 10.1136/bmj.327.7428.1405

Real time assay of Aspergillus should be used in SARS patients receiving corticosteroids

Ya Ping Wu 1,2,3, Ran Wei 1,2,3, Jan Verhoef 1,2,3
PMCID: PMC293004  PMID: 14670903

Editor—No consensus currently exists on treatment of the severe acute respiratory syndrome (SARS). Wong et al reported that all patients with SARS received broad spectrum antibiotics and a combination of ribavirin and prednisolone.1 Intravenous methylprednisolone at high dosage was used in patients with respiratory distress or progressive consolidations in a chest radiograph.

However, the treatment of SARS with ribavarin and corticosteroids remains controversial.2 Corticosteroids are administered to suppress a possible cytokine storm, which may worsen the lung injury caused by the infectious agent.2 But using corticosteroids with possibly ineffective antiviral agents in patients with virus induced pneumonitis can be hazardous.2

If corticosteroids are administered doctors must always be aware of complications such as superinfections with Aspergillus,3 a known complication in any patient receiving corticosteroids.4 Patients with SARS receiving corticosteroids should therefore be monitored for aspergillosis.

Since Aspergillus usually grows slowly on culture (taking up to six days) and is characterised by low sensitivity, we advise introducing an assay using amplification by the polymerase chain reaction, performed in real time, to detect 18SrRNA Aspergillus specific sequences in specimens obtained by bronchoalveolar lavage. Such an assay should be used in association with galactomannan antigen detection by enzyme linked immunosorbent assay (ELISA), as described by Sanguinetti et al.5

This promising method for diagnosing aspergillosis is highly sensitive, fast, specific, and non-invasive. It is certainly less traumatic than lung biopsy.4,5

Competing interests: None declared.

References

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