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. 2010 May 13;31(9):1650–1660. doi: 10.1093/carcin/bgq086

Fig. 2.

Fig. 2.

Corepressor expression and manipulation in prostate cancer cell lines. Panel (A) differences in corepressor mRNA levels, compared with 18S expression, measured by quantitative real time plolymerase chain reaction in PC-3 and DU 145 cells compared with RWPE-1 cells. Each data point represents the mean of three separate experiments amplified in triplicate wells ±SEM (*P < 0.05, **P < 0.01). Panel (B) total cell proteins were subjected to western immunoblotting. The corepressors are indicated on the left with β-actin used as a control. Panel (C) nuclear and cytoplasmic cell proteins were subjected to western immunoblotting. The NRs are indicated on the left with β-actin used as a control. Panel (D) NCOR1 protein expression levels measured in PC-3 shNCOR1 or empty vector (PC-3 VO). Panel (E) heatmap representation of the significant expression changes through the cell cycle in red (upregulated) and green (downregulated) in PC-3 shNCOR1 compared with PC-3 VO cells. Triplicate biological samples were extracted and analyzed in duplicate and significant expression changes calculated (as measured by one-way analysis of variance analyses P < 0.05). The gene names are indicated on the right. The reduced levels of NCOR1 are indicated underneath.