Fig. 7.

Contraction-stimulated transporter translocation after VAMP3 knockdown. a HL-1 cells were co-transfected with VAMP3 siRNA and pCMV-GLUT4myc 24 h after seeding. After a further 32 h the cells were serum depleted for 16 h and subsequently treated with electric-field stimulation (2 Hz) for 30 min. Afterwards, plasmalemmal GLUT4myc (b) and plasmalemmal CD36 (c) was detected by immunostaining. Data are mean values ± SEM of three independent experiments (n=3), with triplicate measurements for each condition. Statistically different from corresponding basal value (*p < 0.05). nc, non-coding