Fig. 2.

hST3Gal-I transgenic mice express the sialyltransferase in the expected tissues. (A) Construct used to inject fertilized eggs to develop the hST3Gal-I transgenic mice. (B) Frozen sections of tissue from hST3Gal-I transgenic mice and wild-type controls stained with the 4B10 antibody which specifically recognizes human ST3Gal-I. Scale bar represent 100 μm. (C) Enzymatic activity of ST3Gal in the indicated tissues from hST3Gal-I transgenic mice and wild-type control. Galβ1-3GalNAcα-pnp was used as the acceptor substrate (see Materials and methods). Assays were carried out in duplicate, which showed a variation of less than 10%