Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1980 Feb;141(2):436–442. doi: 10.1128/jb.141.2.436-442.1980

Expression of glycosidase activities during germination of Dictyostelium discoideum spores.

L S Tisa, D A Cotter
PMCID: PMC293645  PMID: 6767680

Abstract

Several lysosomal glycosidase activities were examined in vitro during heat-induced germination of Dictyostelium discoideum spores and were found not to be coordinately controlled. The level of beta-glucosidase activity increased significantly during the emergence stage of germination. Both alpha-glucosidase and N-acetyl-beta-glucosaminidase activities remained relatively constant until postemergence, when they increased slightly; alpha-mannosidase activity decreased during all stages of germination. The activity of beta-galactosidase increased slightly during spore swelling, fell below the level initially found in spores at zero time, and increased slightly during postemergence. The expression of all of these enzyme activities, except the increase in beta-galactosidase, appeared to require protein synthesis. Spores in the lag phase of germination which were exposed to severe environmental stress were deactivated and exhibited reduced levels of alpha-glucosidase, beta-glucosidase, and N-acetyl-beta-glucosaminidase activities. Prolonged heat activation treatment reduced the levels of lysosomal glycosidase activities in postactivated spores but did not change the subsequent enzyme patterns during the spore-swelling and emergence stages of germination.

Full text

PDF
436

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  2. Coston M. B., Loomis W. F., Jr Isozymes of beta-glucosidase in Dictyostelium discoideum. J Bacteriol. 1969 Dec;100(3):1208–1217. doi: 10.1128/jb.100.3.1208-1217.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cotter D. A., Garnish F. J., Tisa L. S. The physiological effects of restrictive environmental conditions on Dictyostelium discoideum spore germination. Can J Microbiol. 1979 Jan;25(1):24–31. doi: 10.1139/m79-004. [DOI] [PubMed] [Google Scholar]
  4. Cotter D. A., George R. P. Germination and mitochondrial damage in spores of Dictyostelium discoideum following supraoptimal heating. Arch Microbiol. 1975 Apr 7;103(2):163–168. doi: 10.1007/BF00436344. [DOI] [PubMed] [Google Scholar]
  5. Cotter D. A., O'Connell R. W. Activation and killing of Dictyostelium discoideum spores with urea. Can J Microbiol. 1976 Dec;22(12):1751–1755. doi: 10.1139/m76-258. [DOI] [PubMed] [Google Scholar]
  6. Cotter D. A., Raper K. B. Spore germination in Dictyostelium discoideum: trehalase and the requirement for protein synthesis. Dev Biol. 1970 May;22(1):112–128. doi: 10.1016/0012-1606(70)90009-6. [DOI] [PubMed] [Google Scholar]
  7. Eberhart B. M., Beck R. S. Localization of the beta-glucosidases in Neurospora crassa. J Bacteriol. 1970 Feb;101(2):408–417. doi: 10.1128/jb.101.2.408-417.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Giri J. G., Ennis H. L. Developmental changes in RNA and protein synthesis during germination of Dictyostelium discoideum spores. Dev Biol. 1978 Nov;67(1):189–201. doi: 10.1016/0012-1606(78)90308-1. [DOI] [PubMed] [Google Scholar]
  9. Giri J. G., Ennis H. L. Protein and RNA synthesis during spore germination in the cellular slime mold Dictyostelium discoideum. Biochem Biophys Res Commun. 1977 Jul 11;77(1):282–289. doi: 10.1016/s0006-291x(77)80194-0. [DOI] [PubMed] [Google Scholar]
  10. Jones T. H., de Renobales M., Pon N. Cellulases released during the germination of Dictyostelium discoideum spores. J Bacteriol. 1979 Feb;137(2):752–757. doi: 10.1128/jb.137.2.752-757.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Loomis W. F., Jr Acetylglucosaminidase, an early enzyme in the development of Dictyostelium discoideum. J Bacteriol. 1969 Mar;97(3):1149–1154. doi: 10.1128/jb.97.3.1149-1154.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Loomis W. F., Jr Developmental regulation of alpha-mannosidase in Dictyostelium discoideum. J Bacteriol. 1970 Aug;103(2):375–381. doi: 10.1128/jb.103.2.375-381.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Parish R. W. A labile acid phosphatase isozyme associated with the surface of vegetative Dictyostelium discoideum cells. Biochim Biophys Acta. 1976 Oct 22;444(3):802–809. doi: 10.1016/0304-4165(76)90327-5. [DOI] [PubMed] [Google Scholar]
  14. Pugh D., Cawson R. A. The cytochemical localization of acid hydrolases in four common fungi. Cell Mol Biol Incl Cyto Enzymol. 1977;22(1):125–132. [PubMed] [Google Scholar]
  15. Quance J., Ashworth J. M. Enzyme synthesis in the cellular slime mould Dictyostelium discoideum during the differentiation of myxamoebae grown axenically. Biochem J. 1972 Feb;126(3):609–615. doi: 10.1042/bj1260609. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Van Etten J. L., Freer S. N. Simple procedure for disruption of fungal spores. Appl Environ Microbiol. 1978 Mar;35(3):622–623. doi: 10.1128/aem.35.3.622-623.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES