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. 1980 Feb;141(2):528–533. doi: 10.1128/jb.141.2.528-533.1980

Efflux of beta-galactosidase products from Escherichia coli.

R E Huber, J Lytton, E B Fung
PMCID: PMC293655  PMID: 6767683

Abstract

Several different strains of Escherichia coli were grown on a variety of carbon sources under various growth conditions. Lactose was added (usually at mid-log phase), and the concentrations of the products of beta-galactosidase action on this sugar (galactose, glucose, and allolactose) were determined at various times thereafter in the total culture and in the medium. It was found that with each strain, with all carbon sources, and under all of the conditions studied, a very large proportion of the products were found in the medium. Control studies were carried out which showed that these results were not artifacts of the method of separating the cells from the medium. The results also did not arise from the secretion of beta-galactosidase into the medium, from the diffusion of substrates and products into and out of the cells due to leaks in the membrane, or from faults in the method of sugar analysis. In addition, the results showed that there were very high levels of products inside the cells under the conditions used and that the efflux of the products was rapid. The efflux might be energetically advantageous to the cell as well as being a means of storing excess products until needed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ASENSIO C., AVIGAD G., HORECKER B. L. PREFERENTIAL GALACTOSE UTILIZATION IN A MUTANT STRAIN OF E. COLI. Arch Biochem Biophys. 1963 Dec;103:299–309. doi: 10.1016/0003-9861(63)90419-3. [DOI] [PubMed] [Google Scholar]
  2. BURSTEIN C., COHN M., KEPES A., MONOD J. R OLE DU LACTOSE ET DE SES PRODUITS M'ETABOLIQUES DANS L'INDUCTION DE L'OP'ERON LACTOSE CHEZ ESCHERICHIA COLI. Biochim Biophys Acta. 1965 Apr 19;95:634–639. [PubMed] [Google Scholar]
  3. Barkley M. D., Riggs A. D., Jobe A., Burgeois S. Interaction of effecting ligands with lac repressor and repressor-operator complex. Biochemistry. 1975 Apr 22;14(8):1700–1712. doi: 10.1021/bi00679a024. [DOI] [PubMed] [Google Scholar]
  4. COHEN G. N., RICKENBERG H. V. Concentration spécifique réversible des amino acides chez Escherichia coli. Ann Inst Pasteur (Paris) 1956 Nov;91(5):693–720. [PubMed] [Google Scholar]
  5. FRAENKEL D. G., FALCOZ-KELLY F., HORECKER B. L. THE UTILIZATION OF GLUCOSE 6-PHOSPHATE BY GLUCOKINASELESS AND WILD-TYPE STRAINS OF ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1964 Nov;52:1207–1213. doi: 10.1073/pnas.52.5.1207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Flagg J. L., Wilson T. H. A novel type of coupling between proline and galactoside transport in Escherichia coli. Membr Biochem. 1978;1(1-2):61–72. doi: 10.3109/09687687809064159. [DOI] [PubMed] [Google Scholar]
  7. Fowler A. V. High-level production of -galactosidase by Escherichia coli merodiploids. J Bacteriol. 1972 Nov;112(2):856–860. doi: 10.1128/jb.112.2.856-860.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Henderson P. J., Giddens R. A., Jones-Mortimer M. C. Transport of galactose, glucose and their molecular analogues by Escherichia coli K12. Biochem J. 1977 Feb 15;162(2):309–320. doi: 10.1042/bj1620309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Huber R. E., Kurz G., Wallenfels K. A quantitation of the factors which affect the hydrolase and transgalactosylase activities of beta-galactosidase (E. coli) on lactose. Biochemistry. 1976 May 4;15(9):1994–2001. doi: 10.1021/bi00654a029. [DOI] [PubMed] [Google Scholar]
  10. Huber R. E., Wallenfels K., Kurz G. The action of beta-galactosidase (Escherichia coli) on allolactose. Can J Biochem. 1975 Sep;53(9):1035–1038. doi: 10.1139/o75-142. [DOI] [PubMed] [Google Scholar]
  11. Jobe A., Bourgeois S. lac Repressor-operator interaction. VI. The natural inducer of the lac operon. J Mol Biol. 1972 Aug 28;69(3):397–408. doi: 10.1016/0022-2836(72)90253-7. [DOI] [PubMed] [Google Scholar]
  12. Kornberg H. L. Genetics in the study of carbohydrate transport by bacteria. Sixth Griffith Memorial Lecture. J Gen Microbiol. 1976 Sep;96(1):1–16. doi: 10.1099/00221287-96-1-1. [DOI] [PubMed] [Google Scholar]
  13. MUELLER-HILL B., RICKENBERG H. V., WALLENFELS K. SPECIFICITY OF THE INDUCTION OF THE ENZYMES OF THE LAC OPERON IN ESCHERICHIA COLI. J Mol Biol. 1964 Nov;10:303–318. doi: 10.1016/s0022-2836(64)80049-8. [DOI] [PubMed] [Google Scholar]
  14. Postma P. W. Involvement of the phosphotransferase system in galactose transport in Salmonella typhimurium. FEBS Lett. 1976 Jan 1;61(1):49–53. doi: 10.1016/0014-5793(76)80169-x. [DOI] [PubMed] [Google Scholar]
  15. Riggs A. D., Newby R. F., Bourgeois S. lac repressor--operator interaction. II. Effect of galactosides and other ligands. J Mol Biol. 1970 Jul 28;51(2):303–314. doi: 10.1016/0022-2836(70)90144-0. [DOI] [PubMed] [Google Scholar]
  16. Tanaka S., Fraenkel D. G., Lin E. C. The enzymatic lesion of strain MM-6, a pleiotropic carbohydrate-negative mutant of Escherichia coli. Biochem Biophys Res Commun. 1967 Apr 7;27(1):63–67. doi: 10.1016/s0006-291x(67)80040-8. [DOI] [PubMed] [Google Scholar]
  17. Winkler H. H., Wilson T. H. The role of energy coupling in the transport of beta-galactosides by Escherichia coli. J Biol Chem. 1966 May 25;241(10):2200–2211. [PubMed] [Google Scholar]

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