Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1980 Feb;141(2):558–564. doi: 10.1128/jb.141.2.558-564.1980

Yeast mutants auxotrophic for choline or ethanolamine.

K D Atkinson, B Jensen, A I Kolat, E M Storm, S A Henry, S Fogel
PMCID: PMC293659  PMID: 6988386

Abstract

Three mutants of the yeast Saccharomyces cerevisiae which require exogenous ethanolamine or choline were isolated. The mutants map to a single locus (cho1) on chromosome V. The lipid composition suggests that cho1 mutants do not synthesize phosphatidylserine under any growth conditions. If phosphatidylethanolamine or phosphatidylcholine, which are usually derived from phosphatidylserine, were synthesized from exogenous ethanolamine or choline, the mutants grew and divided relatively normally. However, mitochondrial abnormalities were evident even when ethanolamine and choline were supplied. Diploids homozygous for the cho1 mutation were defective in sporulation. Growth on nonfermentable carbon sources was slow, and a high proportion of respiratory-deficient (petite) cells were generated in cho1 cultures.

Full text

PDF
558

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ames G. F. Lipids of Salmonella typhimurium and Escherichia coli: structure and metabolism. J Bacteriol. 1968 Mar;95(3):833–843. doi: 10.1128/jb.95.3.833-843.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cronan J. E., Jr Molecular biology of bacterial membrane lipids. Annu Rev Biochem. 1978;47:163–189. doi: 10.1146/annurev.bi.47.070178.001115. [DOI] [PubMed] [Google Scholar]
  3. Culbertson M. R., Henry S. A. Inositol-requiring mutants of Saccharomyces cerevisiae. Genetics. 1975 May;80(1):23–40. doi: 10.1093/genetics/80.1.23. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DITTMER J. C., LESTER R. L. A SIMPLE, SPECIFIC SPRAY FOR THE DETECTION OF PHOSPHOLIPIDS ON THIN-LAYER CHROMATOGRAMS. J Lipid Res. 1964 Jan;5:126–127. [PubMed] [Google Scholar]
  5. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  6. Fogel S., Hurst D. D. Meiotic gene conversion in yeast tetrads and the theory of recombination. Genetics. 1967 Oct;57(2):455–481. doi: 10.1093/genetics/57.2.455. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fogel S., Mortimer R., Lusnak K., Tavares F. Meiotic gene conversion: a signal of the basic recombination event in yeast. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 2):1325–1341. doi: 10.1101/sqb.1979.043.01.152. [DOI] [PubMed] [Google Scholar]
  8. HANES C. S., ISHERWOOD F. A. Separation of the phosphoric esters on the filter paper chromatogram. Nature. 1949 Dec 31;164(4183):1107-12, illust. doi: 10.1038/1641107a0. [DOI] [PubMed] [Google Scholar]
  9. Hawrot E., Kennedy E. P. Phospholipid composition and membrane function in phosphatidylserine decarboxylase mutants of Escherichia coli. J Biol Chem. 1978 Nov 25;253(22):8213–8220. [PubMed] [Google Scholar]
  10. Hennen P. E., Carter H. B., Nunn W. D. Changes in macromolecular synthesis and nucleoside triphosphate levels during glycerol-induced growth stasis of Escherichia coli. J Bacteriol. 1978 Dec;136(3):929–935. doi: 10.1128/jb.136.3.929-935.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Henry S. A., Atkinson K. D., Kolat A. I., Culbertson M. R. Growth and metabolism of inositol-starved Saccharomyces cerevisiae. J Bacteriol. 1977 Apr;130(1):472–484. doi: 10.1128/jb.130.1.472-484.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Henry S. A., Fogel S. Saturated fatty acid mutants in yeast. Mol Gen Genet. 1971;113(1):1–19. doi: 10.1007/BF00335003. [DOI] [PubMed] [Google Scholar]
  13. Hubbard S. C., Brody S. Glycerophospholipid variation in choline and inositol auxotrophs of Neurospora crassa. Internal compensation among zwitterionic and anionic species. J Biol Chem. 1975 Sep 25;250(18):7173–7181. [PubMed] [Google Scholar]
  14. KENNEDY E. P., WEISS S. B. The function of cytidine coenzymes in the biosynthesis of phospholipides. J Biol Chem. 1956 Sep;222(1):193–214. [PubMed] [Google Scholar]
  15. Lindegren G., Hwang Y. L., Oshima Y., Lindegren C. C. Genetical mutants induced by ethyl methanesulfonate in Saccharomyces. Can J Genet Cytol. 1965 Sep;7(3):491–499. doi: 10.1139/g65-064. [DOI] [PubMed] [Google Scholar]
  16. Linnemans W. A., Boer P., Elbers P. F. Localization of acid phosphatase in Saccharomyces cerevisiae: a clue to cell wall formation. J Bacteriol. 1977 Aug;131(2):638–644. doi: 10.1128/jb.131.2.638-644.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Moor H. Endoplasmic reticulum as the initiator of bud formation in yeast (S. cerevisiae). Arch Mikrobiol. 1967 Jun 6;57(2):135–146. doi: 10.1007/BF00408697. [DOI] [PubMed] [Google Scholar]
  18. Papahadjopoulos D., Portis A., Pangborn W. Calcium-induced lipid phase transitions and membrane fusion. Ann N Y Acad Sci. 1978;308:50–66. doi: 10.1111/j.1749-6632.1978.tb22013.x. [DOI] [PubMed] [Google Scholar]
  19. Perkins D. D. Biochemical Mutants in the Smut Fungus Ustilago Maydis. Genetics. 1949 Sep;34(5):607–626. doi: 10.1093/genetics/34.5.607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Raetz C. R. Enzymology, genetics, and regulation of membrane phospholipid synthesis in Escherichia coli. Microbiol Rev. 1978 Sep;42(3):614–659. doi: 10.1128/mr.42.3.614-659.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Resnick M. A., Mortimer R. K. Unsaturated fatty acid mutants of Saccharomyces cerevisiae. J Bacteriol. 1966 Sep;92(3):597–600. doi: 10.1128/jb.92.3.597-600.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Scarborough G. A., Nyc J. F. Methylation of ethanolamine phosphatides by microsomes from normal and mutant strains of Neurospora crassa. J Biol Chem. 1967 Jan 25;242(2):238–242. [PubMed] [Google Scholar]
  23. Schweizer E., Bolling H. A Saccharomyces cerevisiae mutant defective in saturated fatty acid biosynthesis. Proc Natl Acad Sci U S A. 1970 Oct;67(2):660–666. doi: 10.1073/pnas.67.2.660. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Silbert D. F., Cronan J. E., Jr, Beacham I. R., Harder M. E. Proceedings: Genetic engineering of membrane lipid. Fed Proc. 1974 Jun;33(6):1725–1732. [PubMed] [Google Scholar]
  25. Silbert D. F. Genetic modification of membrane lipid. Annu Rev Biochem. 1975;44:315–339. doi: 10.1146/annurev.bi.44.070175.001531. [DOI] [PubMed] [Google Scholar]
  26. Steiner S., Lester R. L. Metabolism of diphosphoinositide and triphosphoinositide in Saccharomyces cerevisiae. Biochim Biophys Acta. 1972 Jan 27;260(1):82–87. doi: 10.1016/0005-2760(72)90076-8. [DOI] [PubMed] [Google Scholar]
  27. Steiner S., Lester R. L. Studies on the diversity of inositol-containing yeast phospholipids: incorporation of 2-deoxyglucose into lipid. J Bacteriol. 1972 Jan;109(1):81–88. doi: 10.1128/jb.109.1.81-88.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Tyhach R. J., Hawrot E., Satre M., Kennedy E. P. Increased synthesis of phosphatidylserine decarboxylase in a strain of Escherichia coli bearing a hybrid plasmid. Altered association of enzyme with the membrane. J Biol Chem. 1979 Feb 10;254(3):627–633. [PubMed] [Google Scholar]
  29. Waechter C. J., Lester R. L. Differential regulation of the N-methyl transferases responsible for phosphatidylcholine synthesis in Saccharomyces cerevisiae. Arch Biochem Biophys. 1973 Sep;158(1):401–410. doi: 10.1016/0003-9861(73)90637-1. [DOI] [PubMed] [Google Scholar]
  30. Waechter C. J., Lester R. L. Regulation of phosphatidylcholine biosynthesis in Saccharomyces cerevisiae. J Bacteriol. 1971 Mar;105(3):837–843. doi: 10.1128/jb.105.3.837-843.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Waechter C. J., Steiner M. R., Lester R. L. Regulation of phosphatidylcholine biosynthesis by the methylation pathway in Saccharomyces cerevisiae. J Biol Chem. 1969 Jun 25;244(12):3419–3422. [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES