Abstract
The influence of a cloned trpE (trpE+p) gene from Bacillus pumilus on the expression of the gat locus in Bacillus subtilis was examined. The trpE gene was regulated by tryptophan and the mtr locus, which specifies the presumed aporepressor. The specific activity of subunit G varied directly with the level of subunit Ep, and the heterologous EpG complex that was formed was stable to gel filtration.
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Selected References
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- Hoch S. O., Crawford I. P. Enzymes of the tryptophan pathway in three Bacillus species. J Bacteriol. 1973 Nov;116(2):685–693. doi: 10.1128/jb.116.2.685-693.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hoch S. O. Mapping of the 5-methyltryptophan resistance locus in Bacillus subtilis. J Bacteriol. 1974 Jan;117(1):315–317. doi: 10.1128/jb.117.1.315-317.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hoch S. O., Roth C. W., Crawford I. P., Nester E. W. Control of tryptophan biosynthesis by the methyltryptophan resistance gene in Bacillus subtilis. J Bacteriol. 1971 Jan;105(1):38–45. doi: 10.1128/jb.105.1.38-45.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Holmes W. M., Kane J. F. Anthranilate synthase from Bacillus subtilis. The role of a reduced subunit X in aggregate formation and amidotransferase activity. J Biol Chem. 1975 Jun 25;250(12):4462–4469. [PubMed] [Google Scholar]
- Jensen R. A. A biochemical basis for apparent abortive transformation in Bacillus subtilis. Genetics. 1968 Dec;60(4):707–717. doi: 10.1093/genetics/60.4.707. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kane J. F., Holmes W. M., Jensen R. A. Metabolic interlock. The dual function of a folate pathway gene as an extra-operonic gene of tryptophan biosynthesis. J Biol Chem. 1972 Mar 10;247(5):1587–1596. [PubMed] [Google Scholar]
- Kane J. F. Regulation of a common amidotransferase subunit. J Bacteriol. 1977 Nov;132(2):419–425. doi: 10.1128/jb.132.2.419-425.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Keggins K. M., Lovett P. S., Duvall E. J. Molecular cloning of genetically active fragments of Bacillus DNA in Bacillus subtilis and properties of the vector plasmid pUB110. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1423–1427. doi: 10.1073/pnas.75.3.1423. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Keggins K. M., Lovett P. S., Marrero R., Hoch S. O. Insertional inactivation of trpC in cloned Bacillus trp segments: evidence for a polar effect on trpF. J Bacteriol. 1979 Sep;139(3):1001–1006. doi: 10.1128/jb.139.3.1001-1006.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Patel N., Holmes W. M., Kane J. F. Homologous and hybrid complexes of anthranilate synthase from Bacillus species. J Bacteriol. 1974 Jul;119(1):220–227. doi: 10.1128/jb.119.1.220-227.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tanaka T., Kuroda M., Sakaguchi K. Isolation and characterization of four plasmids from Bacillus subtilis. J Bacteriol. 1977 Mar;129(3):1487–1494. doi: 10.1128/jb.129.3.1487-1494.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]