Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1980 Mar;141(3):1024–1030. doi: 10.1128/jb.141.3.1024-1030.1980

Replication of ColE1 plasmid deoxyribonucleic acid in a thermosensitive dnaA mutant of Escherichia coli.

M Abe
PMCID: PMC293771  PMID: 6988397

Abstract

The replication of ColE1 deoxyribonucleic acid (DNA) took place at the restrictive temperature in a dnaA mutant, dnaA167(Ts). It proceeded at a constant rate at 42 degrees C for at least 3 h. The replication was insensitive to rifampin, which blocked replication at the permissive temperature or in the presence of chloramphenicol, even at the restrictive temperature. A linear DNA strand of ColE1 longer than unit genome size was synthesized. The structure of the replicating molecules observed by electron microscopy was mostly sigma shaped, composed of a circle of a unit genome length with a double-stranded tail. These observations suggest that the replication of ColE1 DNA proceeds via a rolling-circle type of structure in the absence of dnaA function.

Full text

PDF
1024

Images in this article

1028Image
on p.1028

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abe M. The replication of prophage P1 DNA. Mol Gen Genet. 1974;132(1):63–72. doi: 10.1007/BF00268231. [DOI] [PubMed] [Google Scholar]
  2. Abe M., Tomizawa J. Chromosome replication in Escherichia coli K12 mutant affected in the process of DNA initiation. Genetics. 1971 Sep;69(1):1–15. doi: 10.1093/genetics/69.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bouvier F., Zinder N. D. Effects of the dnaA thermosensitive mutation of Escherichia coli on bacteriophage fl growth and DNA synthesis. Virology. 1974 Jul;60(1):139–150. doi: 10.1016/0042-6822(74)90371-7. [DOI] [PubMed] [Google Scholar]
  4. Clewell D. B., Evenchik B., Cranston J. W. Direct inhibition of Col E 1 plasmid DNA replication in Escherichia coli by rifampicin. Nat New Biol. 1972 May 3;237(70):29–31. doi: 10.1038/newbio237029a0. [DOI] [PubMed] [Google Scholar]
  5. Collins J., Williams P., Helinski D. R. Plasmid ColE1 DNA replication in Escherichia coli strains temperature-sensitive for DNA replication. Mol Gen Genet. 1975;136(4):273–289. doi: 10.1007/BF00341713. [DOI] [PubMed] [Google Scholar]
  6. Denhardt D. T. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. doi: 10.1016/0006-291x(66)90447-5. [DOI] [PubMed] [Google Scholar]
  7. Eisen H., Pereira da Silva L., Jacob F. The regulation and mechanism of DNA synthesis in bacteriophage lambda. Cold Spring Harb Symp Quant Biol. 1968;33:755–764. doi: 10.1101/sqb.1968.033.01.086. [DOI] [PubMed] [Google Scholar]
  8. Eisenberg S., Griffith J., Kornberg A. phiX174 cistron A protein is a multifunctional enzyme in DNA replication. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3198–3202. doi: 10.1073/pnas.74.8.3198. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Geider K. Molecular aspects of DNA replication in Escherichia coli systems. Curr Top Microbiol Immunol. 1976;74:55–112. doi: 10.1007/978-3-642-66336-9_3. [DOI] [PubMed] [Google Scholar]
  10. Gilbert W., Dressler D. DNA replication: the rolling circle model. Cold Spring Harb Symp Quant Biol. 1968;33:473–484. doi: 10.1101/sqb.1968.033.01.055. [DOI] [PubMed] [Google Scholar]
  11. Goebel W. Studies on the initiation of plasmid DNA replication. Eur J Biochem. 1974 Jan 3;41(1):51–62. doi: 10.1111/j.1432-1033.1974.tb03243.x. [DOI] [PubMed] [Google Scholar]
  12. Hirota Y., Mordoh J., Jacob F. On the process of cellular division in Escherichia coli. 3. Thermosensitive mutants of Escherichia coli altered in the process of DNA initiation. J Mol Biol. 1970 Nov 14;53(3):369–387. doi: 10.1016/0022-2836(70)90072-0. [DOI] [PubMed] [Google Scholar]
  13. Ikeda J. E., Yudelevich A., Hurwitz J. Isolation and characterization of the protein coded by gene A of bacteriophage phiX174 DNA. Proc Natl Acad Sci U S A. 1976 Aug;73(8):2669–2673. doi: 10.1073/pnas.73.8.2669. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Inselburg J., Fuke M. Replicating DNA: structure of colicin factor E1. Science. 1970 Aug 7;169(3945):590–592. doi: 10.1126/science.169.3945.590. [DOI] [PubMed] [Google Scholar]
  15. Inselburg J. Replication of colicin E1 plasmid DNA in minicells from a unique replication initiation site. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2256–2259. doi: 10.1073/pnas.71.6.2256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Koths K., Dressler D. Analysis of the phiX DNA replication cycle by electron microscopy. Proc Natl Acad Sci U S A. 1978 Feb;75(2):605–609. doi: 10.1073/pnas.75.2.605. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kupersztoch Y. M., Helinski D. R. A catenated DNA molecule as an intermediate in the replication of the resistance transfer factor R6K in Escherichia coli. Biochem Biophys Res Commun. 1973 Oct 15;54(4):1451–1459. doi: 10.1016/0006-291x(73)91149-2. [DOI] [PubMed] [Google Scholar]
  18. Novick R. P., Smith K., Sheehy R. J., Murphy E. A catenated intermediate in plasmid replication. Biochem Biophys Res Commun. 1973 Oct 15;54(4):1460–1469. doi: 10.1016/0006-291x(73)91150-9. [DOI] [PubMed] [Google Scholar]
  19. Sakakibara Y., Tomizawa J. I. Replication of colicin E1 plasmid DNA in cell extracts. Proc Natl Acad Sci U S A. 1974 Mar;71(3):802–806. doi: 10.1073/pnas.71.3.802. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sakakibara Y., Tomizawa J. Replication of colicin E1 plasmid DNA in cell extracts. II. Selective synthesis of early replicative intermediates. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1403–1407. doi: 10.1073/pnas.71.4.1403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Schekman R. W., Iwaya M., Bromstrup K., Denhardt D. T. The mechanism of replication of phi X174 single-stranded DNA. 3. An enzymic study of the structure of the replicative form II DNA. J Mol Biol. 1971 Apr 28;57(2):177–199. doi: 10.1016/0022-2836(71)90340-8. [DOI] [PubMed] [Google Scholar]
  22. Skalka A. M. DNA replication--bacteriophage lambda. Curr Top Microbiol Immunol. 1977;78:201–237. [PubMed] [Google Scholar]
  23. Tomizawa J. I., Ohmori H., Bird R. E. Origin of replication of colicin E1 plasmid DNA. Proc Natl Acad Sci U S A. 1977 May;74(5):1865–1869. doi: 10.1073/pnas.74.5.1865. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Tomizawa J., Sakakibara Y., Kakefuda T. Replication of colicin E1 plasmid DNA in cell extracts. Origin and direction of replication. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2260–2264. doi: 10.1073/pnas.71.6.2260. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wickner S. H. DNA replication proteins of Escherichia coli. Annu Rev Biochem. 1978;47:1163–1191. doi: 10.1146/annurev.bi.47.070178.005503. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES