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. 1980 Mar;141(3):1127–1133. doi: 10.1128/jb.141.3.1127-1133.1980

Diversity among B6 strains of Agrobacterium tumefaciens.

S E Hamada, S K Farrand
PMCID: PMC293795  PMID: 7364725

Abstract

A total of 20 laboratory substrains of Agrobacterium tumefaciens strain B6 were compared with respect to six characteristics, including 3-ketolactose production, lysogeny, octopine catabolism, tumorigenic host range, and plasmid content. Within this group of strains diversity was found for all characteristics except 3-ketolactose production. Six substrains were lysogenized with an omega-type phage, whereas one substrain appeared neither sensitive to nor lysogenized with this bacteriophage. All but two substrains catabolized octopine and induced tumors on carrot disks. These 18 substrains harbor deoxyribonucleic acid sequences homologous to pTiB6-806. The two substrains unable to catabolize octopine were nontumorigenic and lacked detectable Ti plasmid sequences. Of the 20 substrains, 13 also contained sequences homologous to the cryptic plasmid pAtB6-806; 2 of the 18 substrains tumorigenic on carrots failed to induce tumors on Kalanchoe leaves. Their inability to induced tumors on this host, could not be correlated with lysogeny, with the presence or absence of pAtB6-806, or with the very large cryptic plasmid recently described. The Ti plasmids from these two strains were indistinguishable from pTiB6-806 by restriction enzyme analysis and could genetically convert a cured A. tumefaciens strain to tumorigenicity on both plant species. The results with these two strains suggest that parameters of tumorigenicity, such as host range, may be controlled by the bacterial chromosome.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BEARDSLEY R. E. Lysogenicity in Agrobacterium tumefaciens. J Bacteriol. 1960 Aug;80:180–187. doi: 10.1128/jb.80.2.180-187.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chilton M. D., Currier T. C., Farrand S. K., Bendich A. J., Gordon M. P., Nester E. W. Agrobacterium tumefaciens DNA and PS8 bacteriophage DNA not detected in crown gall tumors. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3672–3676. doi: 10.1073/pnas.71.9.3672. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chilton M. D., Drummond M. H., Merio D. J., Sciaky D., Montoya A. L., Gordon M. P., Nester E. W. Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesis. Cell. 1977 Jun;11(2):263–271. doi: 10.1016/0092-8674(77)90043-5. [DOI] [PubMed] [Google Scholar]
  4. Chilton M. D., Farrand S. K., Levin R., Nester E. W. RP4 promotion of transfer of a large Agrobacterium plasmid which confers virulence. Genetics. 1976 Aug;83(4):609–618. doi: 10.1093/genetics/83.4.609. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chilton M. D., Montoya A. L., Merlo D. J., Drummond M. H., Nutter R., Gordon M. P., Nester E. W. Restriction endonuclease mapping of a plasmid that confers oncogenicity upon Agrobacterium tumefaciens strain B6-806. Plasmid. 1978 Feb;1(2):254–269. doi: 10.1016/0147-619x(78)90043-4. [DOI] [PubMed] [Google Scholar]
  6. Currier T. C., Nester E. W. Evidence for diverse types of large plasmids in tumor-inducing strains of Agrobacterium. J Bacteriol. 1976 Apr;126(1):157–165. doi: 10.1128/jb.126.1.157-165.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Currier T. C., Nester E. W. Isolation of covalently closed circular DNA of high molecular weight from bacteria. Anal Biochem. 1976 Dec;76(2):431–441. doi: 10.1016/0003-2697(76)90338-9. [DOI] [PubMed] [Google Scholar]
  8. Genetello C., Van Larebeke N., Holsters M., De Picker A., Van Montagu M., Schell J. Ti plasmids of Agrobacterium as conjugative plasmids. Nature. 1977 Feb 10;265(5594):561–563. doi: 10.1038/265561a0. [DOI] [PubMed] [Google Scholar]
  9. Hamada S. E., Luckey J. P., Farrand S. K. R-plasmid-mediated chromosomal gene transfer in Agrobacterium tumefaciens. J Bacteriol. 1979 Jul;139(1):280–286. doi: 10.1128/jb.139.1.280-286.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hamilton R. H., Fall M. Z. The loss of tumor-initiating ability in Agrobacterium tumefaciens by incubation at high temperature. Experientia. 1971 Feb 15;27(2):229–230. doi: 10.1007/BF02145913. [DOI] [PubMed] [Google Scholar]
  11. Heberlein G. T., Lippincott J. A. Enhancement of Agrobacterium tumefaciens infectivity by mitomycin C. J Bacteriol. 1967 Nov;94(5):1470–1474. doi: 10.1128/jb.94.5.1470-1474.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Heberlein G. T., Lippincott J. A. Ultraviolet-induced changes in the infectivity of Agrobacterium tumefaciens. J Bacteriol. 1967 Apr;93(4):1246–1253. doi: 10.1128/jb.93.4.1246-1253.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kerr A., Manigault P., Tempé J. Transfer of virulence in vivo and in vitro in Agrobacterium. Nature. 1977 Feb 10;265(5594):560–561. doi: 10.1038/265560a0. [DOI] [PubMed] [Google Scholar]
  14. Leff J., Beardsley R. E. Action tumorigène de l'acide nucleique d'un bactériophage présent dans les cultures de tissu tumoral de tournesol (Helianthus annus) C R Acad Sci Hebd Seances Acad Sci D. 1970 May 20;270(20):2505–2507. [PubMed] [Google Scholar]
  15. Levin R. A., Farrand S. K., Gordon M. P., Nester E. W. Conjugation in Agrobacterium tumefaciens in the absence of plant tissue. J Bacteriol. 1976 Sep;127(3):1331–1336. doi: 10.1128/jb.127.3.1331-1336.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lippincott B. B., Lippincott J. A. Bacterial attachment to a specific wound site as an essential stage in tumor initiation by Agrobacterium tumefaciens. J Bacteriol. 1969 Feb;97(2):620–628. doi: 10.1128/jb.97.2.620-628.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lippincott J. A., Lippincott B. B. Tumor growth complementation among strains of Agrobacterium. J Bacteriol. 1969 Aug;99(2):496–502. doi: 10.1128/jb.99.2.496-502.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Meyers J. A., Sanchez D., Elwell L. P., Falkow S. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. J Bacteriol. 1976 Sep;127(3):1529–1537. doi: 10.1128/jb.127.3.1529-1537.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Montoya A. L., Chilton M. D., Gordon M. P., Sciaky D., Nester E. W. Octopine and nopaline metabolism in Agrobacterium tumefaciens and crown gall tumor cells: role of plasmid genes. J Bacteriol. 1977 Jan;129(1):101–107. doi: 10.1128/jb.129.1.101-107.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Pootjes C., Stemberger B. Host-phage interaction in Agrobacterium tumefaciens. II. Host strain response to mitomycin C induction. Can J Microbiol. 1974 Mar;20(3):367–370. doi: 10.1139/m74-056. [DOI] [PubMed] [Google Scholar]
  21. Schilperoort R. A., Van Sittert N. J., Schell J. The presence of both phage PS8 and Agrobacterium tumefaciens A 6 DNA base sequences in A 6 -induced sterile crown-gall tissue cultured in vitro. Eur J Biochem. 1973 Feb 15;33(1):1–7. doi: 10.1111/j.1432-1033.1973.tb02647.x. [DOI] [PubMed] [Google Scholar]
  22. Sciaky D., Montoya A. L., Chilton M. D. Fingerprints of Agrobacterium Ti plasmids. Plasmid. 1978 Feb;1(2):238–253. doi: 10.1016/0147-619x(78)90042-2. [DOI] [PubMed] [Google Scholar]
  23. Smith E. F., Townsend C. O. A PLANT-TUMOR OF BACTERIAL ORIGIN. Science. 1907 Apr 26;25(643):671–673. doi: 10.1126/science.25.643.671. [DOI] [PubMed] [Google Scholar]
  24. Sonoki S., Kado C. I. Proteins conferred by the virulence-specifying plasmid of Agrobacterium tumefaciens C-58. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3796–3800. doi: 10.1073/pnas.75.8.3796. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Van Larebeke N., Genetello C., Schell J., Schilperoort R. A., Hermans A. K., Van Montagu M., Hernalsteens J. P. Acquisition of tumour-inducing ability by non-oncogenic agrobacteria as a result of plasmid transfer. Nature. 1975 Jun 26;255(5511):742–743. doi: 10.1038/255742a0. [DOI] [PubMed] [Google Scholar]
  26. Watson B., Currier T. C., Gordon M. P., Chilton M. D., Nester E. W. Plasmid required for virulence of Agrobacterium tumefaciens. J Bacteriol. 1975 Jul;123(1):255–264. doi: 10.1128/jb.123.1.255-264.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Zimmerer R. P., Hamilton R. H., Pootjes C. Isolation and morphology of temperature Agrobacterium tumefaciens bacteriophage. J Bacteriol. 1966 Sep;92(3):746–750. doi: 10.1128/jb.92.3.746-750.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]

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