Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1980 Mar;141(3):1222–1229. doi: 10.1128/jb.141.3.1222-1229.1980

Mapping of insertion mutations in gnd of Escherichia coli with deletions defining the ends of the gene.

R E Wolf Jr, J A Cool
PMCID: PMC293816  PMID: 6245062

Abstract

A genetic map was prepared for gnd, the gene of Escherichia coli which encodes the metabolically regulated 6-phosphogluconate dehydrogenase. Direct selection methods were used for the isolation of mutants with deletions that define the respective ends of gnd. These selections depended on the availability of a defective lysogen in which gnd was present on a lambda h80 dgnd his prophage located at the att phi 80 region of the chromosome. Mutants with deletions entering gnd from the his-distal end were selected as Gnd- TonB- mutants. Mutants with his-proximal gnd deletions were selected as Gnd-, temperature-resistant mutants of a specially prepared stable lysogen. Gnd- mutants were also isolated after mutagenesis with bacteriophage Mu cts61, and genetic tests were used to determine which mutants carry a Mu cts61 prophage in gnd. The deletion mutations were mapped against each other and against the insertion mutations through the use of F' merodiploid strains. The insertion mutations mapped at seven distinct sites in gnd; three mapped under the deletions defining the his-proximal portion of the gene and three mapped with the his-distal deletions.

Full text

PDF
1222

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann B. J., Low K. B., Taylor A. L. Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976 Mar;40(1):116–167. doi: 10.1128/br.40.1.116-167.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berman M. L., Beckwith J. Use of gene fusions to isolate promoter mutants in the transfer RNA gene tyrT of Escherichia coli. J Mol Biol. 1979 May 25;130(3):303–315. doi: 10.1016/0022-2836(79)90543-6. [DOI] [PubMed] [Google Scholar]
  3. Daniell E., Abelson J. Lac messenger RNA in lac Z gene mutants of Escherichia coli caused by insertion of bacteriophage Mu. J Mol Biol. 1973 May 15;76(2):319–322. doi: 10.1016/0022-2836(73)90395-1. [DOI] [PubMed] [Google Scholar]
  4. Daniell E., Roberts R., Abelson J. Mutations in the lactose operon caused by bacteriophage Mu. J Mol Biol. 1972 Aug 14;69(1):1–8. doi: 10.1016/0022-2836(72)90019-8. [DOI] [PubMed] [Google Scholar]
  5. Gottesman S., Beckwith J. R. Directed transposition of the arabinose operon: a technique for the isolation of specialized transducing bacteriophages for any Escherichia coli gene. J Mol Biol. 1969 Aug 28;44(1):117–127. doi: 10.1016/0022-2836(69)90408-2. [DOI] [PubMed] [Google Scholar]
  6. Howe M. M., Bade E. G. Molecular biology of bacteriophage mu. Science. 1975 Nov 14;190(4215):624–632. doi: 10.1126/science.1103291. [DOI] [PubMed] [Google Scholar]
  7. Howe M. M. Prophage deletion mapping of bacteriophage Mu-1. Virology. 1973 Jul;54(1):93–101. doi: 10.1016/0042-6822(73)90118-9. [DOI] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Neubauer Z., Calef E. Immunity phase-shift in defective lysogens: non-mutational hereditary change of early regulation of lambda prophage. J Mol Biol. 1970 Jul 14;51(1):1–13. doi: 10.1016/0022-2836(70)90265-2. [DOI] [PubMed] [Google Scholar]
  10. Russell R. L., Abelson J. N., Landy A., Gefter M. L., Brenner S., Smith J. D. Duplicate genes for tyrosine transfer RNA in Escherichia coli. J Mol Biol. 1970 Jan 14;47(1):1–13. doi: 10.1016/0022-2836(70)90397-9. [DOI] [PubMed] [Google Scholar]
  11. SISTROM W. R. On the physical state of the intracellularly accumulates substrates of beta-galactoside-permease in Escherichia coli. Biochim Biophys Acta. 1958 Sep;29(3):579–587. doi: 10.1016/0006-3002(58)90015-5. [DOI] [PubMed] [Google Scholar]
  12. Shapiro J. A., Adhya S. L. The galactose operon of E. coli K-12. II. A deletion analysis of operon structure and polarity. Genetics. 1969 Jun;62(2):249–264. doi: 10.1093/genetics/62.2.249. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. TAYLOR A. L. BACTERIOPHAGE-INDUCED MUTATION IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1963 Dec;50:1043–1051. doi: 10.1073/pnas.50.6.1043. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wolf R. E., Jr, Fraenkel D. G. Isolation of specialized transducing bacteriophages for gluconate 6-phosphate dehydrogenase (gnd) of Escherichia coli. J Bacteriol. 1974 Feb;117(2):468–476. doi: 10.1128/jb.117.2.468-476.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wolf R. E., Jr, Prather D. M., Shea F. M. Growth-rate-dependent alteration of 6-phosphogluconate dehydrogenase and glucose 6-phosphate dehydrogenase levels in Escherichia coli K-12. J Bacteriol. 1979 Sep;139(3):1093–1096. doi: 10.1128/jb.139.3.1093-1096.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wolf R. E., Jr, Shea F. M. Combined use of strain construction and affinity chromatography in the rapid, high-yield purification of 6-phosphogluconate dehydrogenase from Escherichia coli. J Bacteriol. 1979 Apr;138(1):171–175. doi: 10.1128/jb.138.1.171-175.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES