Abstract
We analyzed a series of germ tube-negative variants isolated from Candida albicans 3153A for deoxyribonucleic acid content. As analyzed by flow microfluorometry, the deoxyribonucleic acid level in these variant strains was 50% of that of the parental strain and equivalent to that of haploid Saccharomyces cerevisiae. This finding was confirmed by comparison of survival rates when exposed to the mutagens ultraviolet light, ethyl methane sulfonate, and methyl methane sulfonate. The diameter of the variant cells as compared to the diameter of the parental 3153A strain showed a relationship similar to that of the diameters of haploid versus diploid S. cerevisiae. These results indicate that those strains may be representative of the imperfect stage of C. albicans.
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Selected References
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- Crissman H. A., Mullaney P. F., Steinkamp J. A. Methods and applications of flow systems for analysis and sorting of mammalian cells. Methods Cell Biol. 1975;9(0):179–246. doi: 10.1016/s0091-679x(08)60076-x. [DOI] [PubMed] [Google Scholar]
- Crissman H. A., Steinkamp J. A. Rapid, simultaneous measurement of DNA, protein, and cell volume in single cells from large mammalian cell populations. J Cell Biol. 1973 Dec;59(3):766–771. doi: 10.1083/jcb.59.3.766. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gilbert M. F., McQuitty D. N., Bailey J. E. Flow microfluorometry study of diauxic batch growth of Saccharomyces cerevisiae. Appl Environ Microbiol. 1978 Oct;36(4):615–617. doi: 10.1128/aem.36.4.615-617.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lee K. L., Buckley H. R., Campbell C. C. An amino acid liquid synthetic medium for the development of mycelial and yeast forms of Candida Albicans. Sabouraudia. 1975 Jul;13(2):148–153. doi: 10.1080/00362177585190271. [DOI] [PubMed] [Google Scholar]
- Olaiya A. F., Sogin S. J. Ploidy determination of Canadida albicans. J Bacteriol. 1979 Dec;140(3):1043–1049. doi: 10.1128/jb.140.3.1043-1049.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Steinkamp J. A., Fulwyler M. J., Coulter J. R., Hiebert R. D., Horney J. L., Mullancy P. F. A new multiparameter separator for microscopic particles and biological cells. Rev Sci Instrum. 1973 Sep;44(9):1301–1310. doi: 10.1063/1.1686375. [DOI] [PubMed] [Google Scholar]
- Wickerham L. J., Kurtzman C. P., Herman A. I. Sexual reproduction in Candida lipolytica. Science. 1970 Feb 20;167(3921):1141–1141. doi: 10.1126/science.167.3921.1141. [DOI] [PubMed] [Google Scholar]
- van der Walt J. P., Pitout M. J. Ploidy differences in Sporobolomyces salmonicolor and Candida albicans. Antonie Van Leeuwenhoek. 1969;35(2):227–231. [PubMed] [Google Scholar]