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. 1980 Mar;141(3):1305–1311. doi: 10.1128/jb.141.3.1305-1311.1980

Isolation and characterization of Neurospora crassa mutants impaired in feedback control of ornithine synthesis.

R L Weiss, C A Lee
PMCID: PMC293828  PMID: 6245066

Abstract

Thirty-two independent mutants were isolated which overcame the proline requirement of pro-3 mutations in Neurospora crassa. The mutations were not revertants, appeared to be allelic, were closely linked or allelic to arg-6, and in strains unable to degrade ornithine no longer suppressed the proline requirement. The suppressor mutations did not alter the levels of biosynthetic or catabolic enzymes, yet allowed accumulation of ornithine. Suppressed strains unable to degrade arginine still produced ornithine (as detected by growth) in arginine-supplemented medium. The results suggest that the suppressor mutants were impaired in the feedback inhibition of ornithine synthesis by arginine. The activity of the appropriate biosynthetic enzyme was less sensitive to inhibition by arginine. The potential usefulness of such mutations is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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