Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1980 Mar;141(3):1428–1431. doi: 10.1128/jb.141.3.1428-1431.1980

Lysine decarboxylase mutants of Escherichia coli: evidence for two enzyme forms.

S H Goldemberg
PMCID: PMC293842  PMID: 6767710

Abstract

Mutants with reduced lysine decarboxylase activity were isolated from an Escherichia coli polyamine auxotroph. These mutants could not produce induced enzyme, showing only a very low level of an apparently constitutive form. Both enzyme forms could be demonstrated in the parental strain.

Full text

PDF
1428

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cunningham-Rundles S., Maas W. K. Isolation, characterization, and mapping of Escherichia coli mutants blocked in the synthesis of ornithine decarboxylase. J Bacteriol. 1975 Nov;124(2):791–799. doi: 10.1128/jb.124.2.791-799.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DAVIS B. D., MINGIOLI E. S. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. doi: 10.1128/jb.60.1.17-28.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dion A. S., Cohen S. S. Polyamine stimulation of nucleic acid synthesis in an uninfected and phage-infected polyamine auxotroph of Escherichia coli K12 (arginine-agmatine ureohydrolase-putrescine-spermidine-lysine-cadaverine). Proc Natl Acad Sci U S A. 1972 Jan;69(1):213–217. doi: 10.1073/pnas.69.1.213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Goldemberg S. H., Algranati I. D. Polyamines and protein synthesis: studies in various polyamine-requiring mutants of Escherichia coli. Mol Cell Biochem. 1977 Jul 5;16(2):71–77. doi: 10.1007/BF01732046. [DOI] [PubMed] [Google Scholar]
  5. Hafner E. W., Tabor C. W., Tabor H. Isolation of a metK mutant with a temperature-sensitive S-adenosylmethionine synthetase. J Bacteriol. 1977 Dec;132(3):832–840. doi: 10.1128/jb.132.3.832-840.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Morris D. R., Fillingame R. H. Regulation of amino acid decarboxylation. Annu Rev Biochem. 1974;43(0):303–325. doi: 10.1146/annurev.bi.43.070174.001511. [DOI] [PubMed] [Google Scholar]
  7. Randerath K. An evaluation of film detection methods for weak beta-emitters, particularly tritium. Anal Biochem. 1970 Mar;34:188–205. doi: 10.1016/0003-2697(70)90100-4. [DOI] [PubMed] [Google Scholar]
  8. SHER I. H., MALLETTE M. F. Purification and study of L-lysine decarboxylase from Escherichia coli B. Arch Biochem Biophys. 1954 Dec;53(2):354–369. doi: 10.1016/0003-9861(54)90417-8. [DOI] [PubMed] [Google Scholar]
  9. Sabo D. L., Boeker E. A., Byers B., Waron H., Fischer E. H. Purification and physical properties of inducible Escherichia coli lysine decarboxylase. Biochemistry. 1974 Feb 12;13(4):662–670. doi: 10.1021/bi00701a005. [DOI] [PubMed] [Google Scholar]
  10. Tabor H., Tabor C. W., Hafner E. W. Convenient method for detecting 14CO2 in multiple samples: application to rapid screening for mutants. J Bacteriol. 1976 Oct;128(1):485–486. doi: 10.1128/jb.128.1.485-486.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES