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. 1980 Apr;142(1):52–59. doi: 10.1128/jb.142.1.52-59.1980

Autolysis of Escherichia coli.

M Leduc, J van Heijenoort
PMCID: PMC293900  PMID: 6768717

Abstract

Autolysis of unwashed exponential-phase Escherichia coli cells was efficiently promoted by first submitting them to a quick downshock with distilled water before an upshock with 0.5 M sodium acetate, pH 6.5. The association of these two osmotic shocks had a remarkable synergistic effect and led to significant decreases in turbidity and viability. Different factors influencing the rate of cell lysis were examined. A close correlation was established between autolysis and the degradation of peptidoglycan. Both phenomena were induced by the same shock treatment, followed similar kinetics, and were efficiently blocked by addition of divalent cations. Cell lysis was also inducible by a shock treatment with 10(-3) M ethylenediaminetetraacetic acid or ethylene glycol-bis(beta-aminoethyl ether)-N,N-tetraacetic acid and blocked by the addition of divalent cations.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRITTEN R. J., McCLURE F. T. The amino acid pool in Escherichia coli. Bacteriol Rev. 1962 Sep;26:292–335. doi: 10.1128/br.26.3.292-335.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Braun V. Covalent lipoprotein from the outer membrane of Escherichia coli. Biochim Biophys Acta. 1975 Oct 31;415(3):335–377. doi: 10.1016/0304-4157(75)90013-1. [DOI] [PubMed] [Google Scholar]
  3. Brunner D. P., Caputo R. A., Treick R. W. Functional reconstitution of EDTA-treated Escherichia coli. Biochem Biophys Res Commun. 1977 Feb 7;74(3):919–925. doi: 10.1016/0006-291x(77)91606-0. [DOI] [PubMed] [Google Scholar]
  4. CHRISTIAN J. H., INGRAM M. Lysis of Vibrio costicolus by osmotic shock. J Gen Microbiol. 1959 Feb;20(1):32–42. doi: 10.1099/00221287-20-1-32. [DOI] [PubMed] [Google Scholar]
  5. DePamphilis M. L. Dissociation and reassembly of Escherichia coli outer membrane and of lipopolysaccharide, and their reassembly onto flagellar basal bodies. J Bacteriol. 1971 Mar;105(3):1184–1199. doi: 10.1128/jb.105.3.1184-1199.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DiRienzo J. M., Nakamura K., Inouye M. The outer membrane proteins of Gram-negative bacteria: biosynthesis, assembly, and functions. Annu Rev Biochem. 1978;47:481–532. doi: 10.1146/annurev.bi.47.070178.002405. [DOI] [PubMed] [Google Scholar]
  7. EAGON R. G., CARSON K. J. LYSIS OF CELL WALLS AND INTACT CELLS OF PSEUDOMONAS AERUGINOSA BY ETHYLENEDIAMINE TETRAACETIC ACID AND BY LYSOZYME. Can J Microbiol. 1965 Apr;11:193–201. doi: 10.1139/m65-025. [DOI] [PubMed] [Google Scholar]
  8. Elmros T., Burman L. G., Bloom G. D. Autolysis of Neisseria gonorrhoeae. J Bacteriol. 1976 May;126(2):969–976. doi: 10.1128/jb.126.2.969-976.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ghuysen J. M. Use of bacteriolytic enzymes in determination of wall structure and their role in cell metabolism. Bacteriol Rev. 1968 Dec;32(4 Pt 2):425–464. [PMC free article] [PubMed] [Google Scholar]
  10. Glaser L. Bacterial cell surface polysaccharides. Annu Rev Biochem. 1973;42:91–112. doi: 10.1146/annurev.bi.42.070173.000515. [DOI] [PubMed] [Google Scholar]
  11. Hartmann R., Bock-Hennig S. B., Schwarz U. Murein hydrolases in the envelope of Escherichia coli. Properties in situ and solubilization from the envelope. Eur J Biochem. 1974 Jan 3;41(1):203–208. doi: 10.1111/j.1432-1033.1974.tb03261.x. [DOI] [PubMed] [Google Scholar]
  12. Hebeler B. H., Young F. E. Autolysis of Neisseria gonorrhoeae. J Bacteriol. 1975 May;122(2):385–392. doi: 10.1128/jb.122.2.385-392.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hirota Y., Suzuki H., Nishimura Y., Yasuda S. On the process of cellular division in Escherichia coli: a mutant of E. coli lacking a murein-lipoprotein. Proc Natl Acad Sci U S A. 1977 Apr;74(4):1417–1420. doi: 10.1073/pnas.74.4.1417. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hughes R. C. Autolysis of isolated cell walls of Bacillus licheniformis N.C.T.C. 6346 and Bacillus subtilis Marburg Strain 168. Separation of the products and characterization of the mucopeptide fragments. Biochem J. 1970 Oct;119(5):849–860. doi: 10.1042/bj1190849. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Iwaya M., Strominger J. L. Simultaneous deletion of D-alanine carboxypeptidase IB-C and penicillin-binding component IV in a mutant of Escherichia coli K12. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2980–2984. doi: 10.1073/pnas.74.7.2980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kung F. C., Raymond J., Glaser D. A. Metal ion content of Escherichia coli versus cell age. J Bacteriol. 1976 Jun;126(3):1089–1095. doi: 10.1128/jb.126.3.1089-1095.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Leive L. Release of lipopolysaccharide by EDTA treatment of E. coli. Biochem Biophys Res Commun. 1965 Nov 22;21(4):290–296. doi: 10.1016/0006-291x(65)90191-9. [DOI] [PubMed] [Google Scholar]
  18. Leive L. Studies on the permeability change produced in coliform bacteria by ethylenediaminetetraacetate. J Biol Chem. 1968 May 10;243(9):2373–2380. [PubMed] [Google Scholar]
  19. Leutgeb W., Schwarz U. Zur Biosynthese des formgebenden Elements der Bakterienzellwand. I. Abbau des Mureins als erster Schritt beim Wachstum des Sacculus. Z Naturforsch B. 1967 May;22(5):545–549. [PubMed] [Google Scholar]
  20. MACLEOD R. A. THE QUESTION OF THE EXISTENCE OF SPECIFIC MARINE BACTERIA. Bacteriol Rev. 1965 Mar;29:9–24. [PMC free article] [PubMed] [Google Scholar]
  21. Matsuhashi M., Takagaki Y., Maruyama I. N., Tamaki S., Nishimura Y., Suzuki H., Ogino U., Hirota Y. Mutants of Escherichia coli lacking in highly penicillin-sensitive D-alanine carboxypeptidase activity. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2976–2979. doi: 10.1073/pnas.74.7.2976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Mohan R. R., Kronish D. P., Pianotti R. S., Epstein R. L., Schwartz B. S. Autolytic mechanism for spheroplast formation in Bacillus cereus and Escherichia coli. J Bacteriol. 1965 Nov;90(5):1355–1364. doi: 10.1128/jb.90.5.1355-1364.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Rayman M. K., MacLeod R. A. Interaction of Mg-2+ with peptidoglycan and its relation to the prevention of lysis of a marine pseudomonad. J Bacteriol. 1975 May;122(2):650–659. doi: 10.1128/jb.122.2.650-659.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Rogers H. J. Bacterial growth and the cell envelope. Bacteriol Rev. 1970 Jun;34(2):194–214. doi: 10.1128/br.34.2.194-214.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. SCHWARTZ U., WEIDEL W. ZUM WIRKUNGSMECHANISMUS VON PENICILLIN. II. NACHWEIS EINES PENICILLIN-INDUZIERTEN HYDROLYTISCHEN ABBUS VON MUREIN UND MUREINVORSTUFEN IN E. COLI B. Z Naturforsch B. 1965 Feb;20:153–157. [PubMed] [Google Scholar]
  26. Shockman G. D. Symposium on the fine structure and replication of bacteria and their parts. IV. Unbalanced cell-wall synthesis: autolysis and cell-wall thickening. Bacteriol Rev. 1965 Sep;29(3):345–358. doi: 10.1128/br.29.3.345-358.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Suzuki H., Nishimura Y., Yasuda S., Nishimura A., Yamada M., Hirota Y. Murein-lipoprotein of Escherichia coli: a protein involved in the stabilization of bacterial cell envelope. Mol Gen Genet. 1978 Nov 16;167(1):1–9. doi: 10.1007/BF00270315. [DOI] [PubMed] [Google Scholar]
  28. Wegener W. S., Hebeler B. H., Morse S. A. Cell envelope of Neisseria gonorrhoeae: relationship between autolysis in buffer and the hydrolysis of peptidoglycan. Infect Immun. 1977 Oct;18(1):210–219. doi: 10.1128/iai.18.1.210-219.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Yem D. W., Wu H. C. Isolation of Escherichia coli K-12 mutants with altered level of beta-N-acetylglucosaminidase. J Bacteriol. 1976 Jan;125(1):372–373. doi: 10.1128/jb.125.1.372-373.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Young F. E. Autolytic enzyme associated with cell walls of Bacillus subtilis. J Biol Chem. 1966 Aug 10;241(15):3462–3467. [PubMed] [Google Scholar]
  31. van Heijenoort J., Parquet C., Flouret B., van Heijenoort Y. Envelope-bound N-acetylmuramyl-L-alanine amidase of Escherichia coli K 12. Purification and properties of the enzyme. Eur J Biochem. 1975 Oct 15;58(2):611–619. doi: 10.1111/j.1432-1033.1975.tb02412.x. [DOI] [PubMed] [Google Scholar]

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