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. Author manuscript; available in PMC: 2011 Sep 21.
Published in final edited form as: Langmuir. 2010 Sep 21;26(18):15050–15056. doi: 10.1021/la102306z

Figure 5.

Figure 5

Thickness profile of each layer of PSA-SWNT immunoassembly determined using three-dimensional non-contact scanning white light interferometer (O) and quartz crystal microbalance (●). 1. Nafion was first deposited on the glass substrate followed by; 2. Nafion/ FeO(OH)-FeOCl bilayer; 3. SWNT forests on Nafion/ FeO(OH)-FeOCl bilayer; 4. 400 mM EDC and 100 mM NHSS in water; 5. after covalent linkage of 2 nmol mL−1 primary anti-PSA antibody (Ab1) in 0.05% Tween-20 PBS buffer to SWNT forests; 6. adsorption of 2% BSA in 0.05% Tween-20 PBS buffer onto antibody; 7. adsorption of 40 ng mL−1 PSA in calf serum; 8. 4 pmol mL−1 Ab2-HRP in PBS containing 0.05% Tween-20.