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. Author manuscript; available in PMC: 2011 Oct 25.

Published in final edited form as: Virology. 2010 Aug 7;406(2):202–211. doi: 10.1016/j.virol.2010.07.018

Fig. 5 — Upregulation of interferon-stimulated genes (ISGs) by TAg. (A) Transcript levels of ISGs were analyzed in normal MEFs and MEFs expressing TAg^wt (TAg), TAg^N136 (N136), TAg³²¹³ (3213) and TAg^D44N (D44N). Two independent clones of each cell line were used for this experiment. cDNAs were reverse-transcribed from equal amounts of total RNA and subjected to PCR using specific primers. Transcript level of alcohol dehydrogenase 5 (Adh5) was used as a loading control. (B) MEFs were infected with SV40 (lane 2) or stably expressing TAg or its mutants (lane 3–6) were used. Whole-cell extracts from these cells were subjected to immunoblot for ISG54. Mock (lane 1) represent normal MEFs. β-tubulin was used as a loading control. (C) Whole cell extract from BJ cells (human fibroblast) expressing empty vector or TAg were subjected to immunoblots for ISG56 and IRF-9. β-tubulin was used as a loading control.

Fig. 5 — Upregulation of interferon-stimulated genes (ISGs) by TAg. (A) Transcript levels of ISGs were analyzed in normal MEFs and MEFs expressing TAg^wt (TAg), TAg^N136 (N136), TAg³²¹³ (3213) and TAg^D44N (D44N). Two independent clones of each cell line were used for this experiment. cDNAs were reverse-transcribed from equal amounts of total RNA and subjected to PCR using specific primers. Transcript level of alcohol dehydrogenase 5 (Adh5) was used as a loading control. (B) MEFs were infected with SV40 (lane 2) or stably expressing TAg or its mutants (lane 3–6) were used. Whole-cell extracts from these cells were subjected to immunoblot for ISG54. Mock (lane 1) represent normal MEFs. β-tubulin was used as a loading control. (C) Whole cell extract from BJ cells (human fibroblast) expressing empty vector or TAg were subjected to immunoblots for ISG56 and IRF-9. β-tubulin was used as a loading control.