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. 1980 May;142(2):400–406. doi: 10.1128/jb.142.2.400-406.1980

A mutation, adjacent to gene amdS, defining the site of action of positive-control gene amdR in Aspergillus nidulans.

M J Hynes
PMCID: PMC293985  PMID: 6769897

Abstract

In Aspergillus nidulans the acetamidase enzyme is inducible by omega-amino acids, sources of acetyl-coenzyme A, and benzoate. The amdR (or intA) gene is a positive-control gene involved in omega-amino acid induction only. A cis-acting mutation amdI93 located in a complex controlling region adjacent to the acetamidase structural gene was found to abolish induction by omega-amino acids but not induction by other sources of induction. As predicted, this mutation was epistatic to constitutive amdR alleles but did not affect the expression of mutations in other regulatory genes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arst H. N., Jr, Cove D. J. Nitrogen metabolite repression in Aspergillus nidulans. Mol Gen Genet. 1973 Nov 2;126(2):111–141. doi: 10.1007/BF00330988. [DOI] [PubMed] [Google Scholar]
  2. Arst H. N., Jr Integrator gene in Aspergillus nidulans. Nature. 1976 Jul 15;262(5565):231–234. doi: 10.1038/262231a0. [DOI] [PubMed] [Google Scholar]
  3. Arst H. N., Jr Some genetical aspects of ornithine metabolism in Aspergillus nidulans. Mol Gen Genet. 1977 Feb 28;151(1):105–110. doi: 10.1007/BF00446919. [DOI] [PubMed] [Google Scholar]
  4. Beckwith J., Rossow P. Analysis of genetic regulatory mechanisms. Annu Rev Genet. 1974;8:1–13. doi: 10.1146/annurev.ge.08.120174.000245. [DOI] [PubMed] [Google Scholar]
  5. Britten R. J., Davidson E. H. Gene regulation for higher cells: a theory. Science. 1969 Jul 25;165(3891):349–357. doi: 10.1126/science.165.3891.349. [DOI] [PubMed] [Google Scholar]
  6. Cove D. J. The induction and repression of nitrate reductase in the fungus Aspergillus nidulans. Biochim Biophys Acta. 1966 Jan 11;113(1):51–56. doi: 10.1016/s0926-6593(66)80120-0. [DOI] [PubMed] [Google Scholar]
  7. Dunsmuir P., Hynes M. J. Temperature sensitive mutants affecting the activity and regulation of the acetamidase of Aspergillus nidulans. Mol Gen Genet. 1973 Jul 16;123(4):333–346. doi: 10.1007/BF00433650. [DOI] [PubMed] [Google Scholar]
  8. Gielow L., Largen M., Englesberg E. Initiator constitutive mutants of the L-arabinose operon (OIBAD) of Escherichia coli B/r. Genetics. 1971 Nov;69(3):289–302. doi: 10.1093/genetics/69.3.289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hynes M. J. A cis-dominant regulatory mutation affecting enzyme induction in the eukaryote Aspergillus nidulans. Nature. 1975 Jan 17;253(5488):210–212. doi: 10.1038/253210a0. [DOI] [PubMed] [Google Scholar]
  10. Hynes M. J. An "up-promotor" mutation affecting the acetamidase of Aspergillus nidulans. Mol Gen Genet. 1978 Oct 25;166(1):31–36. doi: 10.1007/BF00379726. [DOI] [PubMed] [Google Scholar]
  11. Hynes M. J. Effects of ammonium, L-glutamate, and L-glutamine on nitrogen catabolism in Aspergillus nidulans. J Bacteriol. 1974 Dec;120(3):1116–1123. doi: 10.1128/jb.120.3.1116-1123.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hynes M. J. Fine-structure mapping of the acetamidase structural gene and its controlling region in Aspergillus nidulans. Genetics. 1979 Mar;91(3):381–392. doi: 10.1093/genetics/91.3.381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hynes M. J. Induction of the acetamidase of Aspergillus nidulans by acetate metabolism. J Bacteriol. 1977 Sep;131(3):770–775. doi: 10.1128/jb.131.3.770-775.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hynes M. J. Multiple independent control mechanisms affecting the acetamidase of Aspergillus nidulans. Mol Gen Genet. 1978 Apr 25;161(1):59–65. doi: 10.1007/BF00266615. [DOI] [PubMed] [Google Scholar]
  15. Hynes M. J. Mutants with altered glucose repression of amidase enzymes in Aspergillus nidulans. J Bacteriol. 1972 Sep;111(3):717–722. doi: 10.1128/jb.111.3.717-722.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Hynes M. J., Pateman J. A. The genetic analysis of regulation of amidase synthesis in Aspergillus nidulans. I. Mutants able to utilize acrylamide. Mol Gen Genet. 1970;108(2):97–106. doi: 10.1007/BF02430516. [DOI] [PubMed] [Google Scholar]
  17. Hynes M. J., Pateman J. A. The genetic analysis of regulation of amidase synthesis in Aspergillus nidulans. II. Mutants resistant to fluoroacetamide. Mol Gen Genet. 1970;108(2):107–116. doi: 10.1007/BF02430517. [DOI] [PubMed] [Google Scholar]
  18. Hynes M. J. Pleiotropic mutants affecting the control of nitrogen metabolism in Aspergillus nidulans. Mol Gen Genet. 1973 Sep 5;125(2):99–107. doi: 10.1007/BF00268862. [DOI] [PubMed] [Google Scholar]
  19. Hynes M. J. Studies on the role of the areA gene in the regulation of nitrogen catabolism in Aspergillus nidulans. Aust J Biol Sci. 1975 Jun;28(3):301–313. doi: 10.1071/bi9750301. [DOI] [PubMed] [Google Scholar]

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