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. 1980 May;142(2):568–580. doi: 10.1128/jb.142.2.568-580.1980

Interactions of competent Streptococcus sanguis (Wicky) cells with native or denatured, homologous or heterologous deoxyribonucleic acids.

J M Ranhand
PMCID: PMC294025  PMID: 6991480

Abstract

Competent cell-deoxyribonucleic acid (DNA) interactions were examined using tritium-labeled homologous or heterologous native or denatured DNAs and competent Streptococcus sanguis Wicky cells (strain WE4). The DNAs used were extracted from WE4 cells, Escherichia coli B cells, and E. coli bacteriophages T2, T4, T6, and T7. The reactions examined were: (i) total DNA binding, (ii) deoxyribonuclease-resistant DNA binding, and (iii) the production of acid-soluble products from the DNA. Optimal temperatures for the reactions were as follows: reaction (i), between 30 and 40 degrees C; reaction (ii), 30 degrees C; and reaction (iii), greater than 40 degrees C. The rates for the reactions (expressed as molecules of DNA that reacted per minute per colony-forming unit) did not vary greatly from one DNA source to another. With a constant competent cell concentration and differing DNA concentrations below a saturation level (from a given source), a different but constant fraction of the added DNA was cell bound, deoxyribonuclease resistant, and degraded to acid-soluble products. In experiments where the number of competent cells was varied and the DNA concentration was held constant, again essentially the same result was obtained. The extent of reactions (i), (ii), and (iii) depended upon the numbers as well as the source of DNA molecules applied to competent cells. Calcium ion essential for native DNA-cell reactions was also found essential for denatured DNA-cell reactions. Data obtained from competition experiments lead to the conclusion that competent WE4 cells contain specific sites for native as well as denatured DNAs.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARNHART B. J., HERRIOTT R. M. PENETRATION OF DEOXYRIBONUCLEIC ACID INTO HEMOPHILUS INFLUENZAE. Biochim Biophys Acta. 1963 Sep 17;76:25–39. [PubMed] [Google Scholar]
  2. Benzinger R., Delius H., Janenisch R., Hofschneider P. H. Infectious nucleic acids of Escherichia coli bacteriophages. 10. Preparation and properties of Escherichia coli competent for infectious DNA from bacteriophages phi X 174 and M 13 and RNA from bacteriophage M 12. Eur J Biochem. 1967 Nov;2(4):414–428. doi: 10.1111/j.1432-1033.1967.tb00154.x. [DOI] [PubMed] [Google Scholar]
  3. Ceglowski P., Fuchs P. G., Soltyk A. Competitive inhibition of transformation in group H Streptococcus strain Challis by heterologous deoxyribonucleic acid. J Bacteriol. 1975 Dec;124(3):1621–1623. doi: 10.1128/jb.124.3.1621-1623.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dougherty T. J., Asmus A., Tomasz A. Specificity of DNA uptake in genetic transformation of gonococci. Biochem Biophys Res Commun. 1979 Jan 15;86(1):97–104. doi: 10.1016/0006-291x(79)90386-3. [DOI] [PubMed] [Google Scholar]
  5. Eisenstadt E., Lange R., Willecke K. Competent Bacillus subtilis cultures synthesize a denatured DNA binding activity. Proc Natl Acad Sci U S A. 1975 Jan;72(1):323–327. doi: 10.1073/pnas.72.1.323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. FOX M. S., HOTCHKISS R. D. Initiation of bacterial transformation. Nature. 1957 Jun 29;179(4574):1322–1325. doi: 10.1038/1791322a0. [DOI] [PubMed] [Google Scholar]
  7. GOODGAL S. H., HERRIOTT R. M. Studies on transformations of Hemophilus influenzae. I. Competence. J Gen Physiol. 1961 Jul;44:1201–1227. doi: 10.1085/jgp.44.6.1201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gryczan T. J., Contente S., Dubnau D. Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis. J Bacteriol. 1978 Apr;134(1):318–329. doi: 10.1128/jb.134.1.318-329.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Haseltine F. P., Fox M. S. Bacterial inactivation of transforming deoxyribonucleate. J Bacteriol. 1971 Sep;107(3):889–899. doi: 10.1128/jb.107.3.889-899.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. LACKS S. Molecular fate of DNA in genetic transformation of Pneumococcus. J Mol Biol. 1962 Jul;5:119–131. doi: 10.1016/s0022-2836(62)80067-9. [DOI] [PubMed] [Google Scholar]
  11. LERMAN L. S., TOLMACH L. J. Genetic transformation. I. Cellular incorporation of DNA accompanying transformation in Pneumococcus. Biochim Biophys Acta. 1957 Oct;26(1):68–82. doi: 10.1016/0006-3002(57)90055-0. [DOI] [PubMed] [Google Scholar]
  12. Lacks S., Greenberg B. Competence for deoxyribonucleic acid uptake and deoxyribonuclease action external to cells in the genetic transformation of Diplococcus pneumoniae. J Bacteriol. 1973 Apr;114(1):152–163. doi: 10.1128/jb.114.1.152-163.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lacks S., Greenberg B. Single-strand breakage on binding of DNA to cells in the genetic transformation of Diplococcus pneumoniae. J Mol Biol. 1976 Feb 25;101(2):255–275. doi: 10.1016/0022-2836(76)90376-4. [DOI] [PubMed] [Google Scholar]
  14. Lacks S. Mutants of Diplococcus pneumoniae that lack deoxyribonucleases and other activities possibly pertinent to genetic transformation. J Bacteriol. 1970 Feb;101(2):373–383. doi: 10.1128/jb.101.2.373-383.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lacks S., Neuberger M. Membrane location of a deoxyribonuclease implicated in the genetic transformation of Diplococcus pneumoniae. J Bacteriol. 1975 Dec;124(3):1321–1329. doi: 10.1128/jb.124.3.1321-1329.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lacks S. Uptake of circular deoxyribonucleic acid and mechanism of deoxyribonucleic acid transport in genetic transformation of Streptococcus pneumoniae. J Bacteriol. 1979 May;138(2):404–409. doi: 10.1128/jb.138.2.404-409.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. LeBlanc D. J., Hassell F. P. Transformation of Streptococcus sanguis Challis by plasmid deoxyribonucleic acid from Streptococcus faecalis. J Bacteriol. 1976 Oct;128(1):347–355. doi: 10.1128/jb.128.1.347-355.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Leonard C. G., Ranhand J. M., Cole R. M. Competence factor production in chemically defined media by noncompetent cells of group H Streptococcus strain Challis. J Bacteriol. 1970 Nov;104(2):674–683. doi: 10.1128/jb.104.2.674-683.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Macrina F. L., Reider J. L., Virgili S. S., Kopecko D. J. Survey of the extrachromosomal gene pool of Streptococcus mutans. Infect Immun. 1977 Jul;17(1):215–226. doi: 10.1128/iai.17.1.215-226.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Newman C. M., Stuy J. H. Fate of bacteriophage lambda DNA after adsorption by Haemophilus influenzae. J Gen Microbiol. 1971 Feb;65(2):153–159. doi: 10.1099/00221287-65-2-153. [DOI] [PubMed] [Google Scholar]
  21. Parsons C. L., Cole R. M. Transfection of group H streptococci. J Bacteriol. 1973 Mar;113(3):1505–1506. doi: 10.1128/jb.113.3.1505-1506.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Porter R. D., Guild W. R. Transfection in pneumococcus: single-strand intermediates in the formation of infective centers. J Virol. 1978 Jan;25(1):60–72. doi: 10.1128/jvi.25.1.60-72.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Raina J. L., Ravin A. W. Fate of homospecific transforming DNA bound to Streptococcus sanguis. J Bacteriol. 1978 Mar;133(3):1212–1223. doi: 10.1128/jb.133.3.1212-1223.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Ranhand J. M. Autolytic activity and its association with the development of competence in group H streptococci. J Bacteriol. 1973 Aug;115(2):607–614. doi: 10.1128/jb.115.2.607-614.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Ranhand J. M. Effect of pH on competence development and deoxyribonucleic acid uptake in Streptococcus sanguis (Wicky). J Bacteriol. 1976 Apr;126(1):205–212. doi: 10.1128/jb.126.1.205-212.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Ranhand J. M. Inhibition of the development of competence in Streptococcus sanguis (Wicky) by reagents that interact with sulfhydryl groups: discernment of the competence process. J Bacteriol. 1974 Jun;118(3):1041–1050. doi: 10.1128/jb.118.3.1041-1050.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Ranhand J. M., Leonard C. G., Cole R. M. Autolytic activity associated with competent group H streptococci. J Bacteriol. 1971 Apr;106(1):257–268. doi: 10.1128/jb.106.1.257-268.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. STUDIER F. W. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA. J Mol Biol. 1965 Feb;11:373–390. doi: 10.1016/s0022-2836(65)80064-x. [DOI] [PubMed] [Google Scholar]
  29. STUY J. H., STERN D. THE KINETICS OF DNA UPTAKE BY HAEMOPHILUS INFLUENZAE. J Gen Microbiol. 1964 Jun;35:391–400. doi: 10.1099/00221287-35-3-391. [DOI] [PubMed] [Google Scholar]
  30. Scher B., Dubnau D. A manganese-stimulated endonuclease from Bacillus subtilis. Biochem Biophys Res Commun. 1973 Dec 10;55(3):595–602. doi: 10.1016/0006-291x(73)91185-6. [DOI] [PubMed] [Google Scholar]
  31. Scocca J. J., Poland R. L., Zoon K. C. Specificity in deoxyribonucleic acid uptake by transformable Haemophilus influenzae. J Bacteriol. 1974 May;118(2):369–373. doi: 10.1128/jb.118.2.369-373.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Seto H., Lopez R., Garrigan O., Tomasz A. Nucleolytic degradation of homologous and heterologous deoxyribonucleic acid molecules at the surface of competent pneumococci. J Bacteriol. 1975 May;122(2):676–685. doi: 10.1128/jb.122.2.676-685.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Seto H., Lopez R., Tomasz A. Cell surface-located deoxyribonucleic acid receptors in transformable pneumococci. J Bacteriol. 1975 Jun;122(3):1339–1350. doi: 10.1128/jb.122.3.1339-1350.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Seto H., Tomasz A. Calcium-requiring step in the uptake of deoxyribonucleic acid molecules through the surface of competent pneumococci. J Bacteriol. 1976 Jun;126(3):1113–1118. doi: 10.1128/jb.126.3.1113-1118.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Seto H., Tomasz A. Early stages in DNA binding and uptake during genetic transformation of pneumococci. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1493–1498. doi: 10.1073/pnas.71.4.1493. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Seto H., Tomasz A. Protoplast formation and leakage of intramembrane cell components: induction by the competence activator substance of pneumococci. J Bacteriol. 1975 Jan;121(1):344–353. doi: 10.1128/jb.121.1.344-353.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Singh R. N. Number of deoxyribonucleic acid uptake sites in competent cells of Bacillus subtilis. J Bacteriol. 1972 Apr;110(1):266–272. doi: 10.1128/jb.110.1.266-272.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Sisco K. L., Smith H. O. Sequence-specific DNA uptake in Haemophilus transformation. Proc Natl Acad Sci U S A. 1979 Feb;76(2):972–976. doi: 10.1073/pnas.76.2.972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Soltyk A., Shugar D., Piechowska M. Heterologous deoxyribonucleic acid uptake and complexing with cellular constituents in competent Bacillus subtilis. J Bacteriol. 1975 Dec;124(3):1429–1438. doi: 10.1128/jb.124.3.1429-1438.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Steinhart W. L., Herriott R. M. Genetic integration in the heterospecific transformation of Haemophilus influenzae cells by Haemophilus parainfluenzae deoxyribonucleic acid. J Bacteriol. 1968 Nov;96(5):1725–1731. doi: 10.1128/jb.96.5.1725-1731.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Stuy J. H. Fate of transforming DNA in the Haemophilus influenzae transformation system. J Mol Biol. 1965 Sep;13(2):554–570. doi: 10.1016/s0022-2836(65)80117-6. [DOI] [PubMed] [Google Scholar]
  42. YOUNG F. E., SPIZIZEN J. INCORPORATION OF DEOXYRIBONUCLEIC ACID IN THE BACILLUS SUBTILIS TRANSFORMATION SYSTEM. J Bacteriol. 1963 Sep;86:392–400. doi: 10.1128/jb.86.3.392-400.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]

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