Abstract
Cell-free extracts from phaseolotoxin-producing strains of Pseudomonas syringae pv. phaseolicola grown at 18 degrees C, the optimum temperature for phaseolotoxin production, contain an ornithine carbamoyltransferase activity that is insensitive to phaseolotoxin. Extracts from the same strains grown at 30 degrees C, a temperature at which little or no detectable phaseolotoxin is produced, and from phaseolotoxin-nonproducing strains contain a phaseolotoxin-sensitive ornithine carbamoyltransferase activity. The phaseolotoxin-insensitive ornithine carbamoyltransferase activity is also less senstive to N delta-(phosphonacetyl)-L-ornithine than the phaseolotoxin-sensitive ornithine carbamoyltransferase activity of the corresponding strain.
Full text
PDF



Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- AMES B. N., DUBIN D. T. The role of polyamines in the neutralization of bacteriophage deoxyribonucleic acid. J Biol Chem. 1960 Mar;235:769–775. [PubMed] [Google Scholar]
- Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
- Hoogenraad N. J. Synthesis and properties of delta-N-(phosphonacetyl)-L-ornithine. A transition-state analog inhibitor of ornithine transcarbamylase. Arch Biochem Biophys. 1978 May;188(1):137–144. doi: 10.1016/0003-9861(78)90366-1. [DOI] [PubMed] [Google Scholar]
- Kwok O. C., Ako H., Patil S. S. Inactivation of bean ornithine carbamoyltransferase by phaseotoxin: effect of phosphate. Biochem Biophys Res Commun. 1979 Aug 28;89(4):1361–1368. doi: 10.1016/0006-291x(79)92159-4. [DOI] [PubMed] [Google Scholar]
- Mitchell R. E., Bieleski R. L. Involvement of phaseolotoxin in halo blight of beans: transport and conversion to functional toxin. Plant Physiol. 1977 Nov;60(5):723–729. doi: 10.1104/pp.60.5.723. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mori M., Aoyagi K., Tatibana M., Ishikawa T., Ishii H. N delta-(phosphonacetyl)-L-ornithine, a potent transition state analogue inhibitor of ornithine carbamoyltransferase. Biochem Biophys Res Commun. 1977 Jun 6;76(3):900–904. doi: 10.1016/0006-291x(77)91586-8. [DOI] [PubMed] [Google Scholar]
- Patil S. S., Youngblood P., Christiansen P., Moore R. E. Phaseotoxin A: an antimetabolite from Pseudomonas phaseolicola. Biochem Biophys Res Commun. 1976 Apr 19;69(4):1019–1027. doi: 10.1016/0006-291x(76)90474-5. [DOI] [PubMed] [Google Scholar]
- Sands D. C., Schroth M. N., Hildebrand D. C. Taxonomy of phytopathogenic pseudomonads. J Bacteriol. 1970 Jan;101(1):9–23. doi: 10.1128/jb.101.1.9-23.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stalon V., Ramos F., Piérard A., Wiame J. M. The occurrence of a catabolic and an anabolic ornithine carbamoyltransferase in Pseudomonas. Biochim Biophys Acta. 1967 May 16;139(1):91–97. doi: 10.1016/0005-2744(67)90115-5. [DOI] [PubMed] [Google Scholar]
- Staskawicz B. J., Panopoulos N. J. Phaseolotoxin transport in Escherichia coli and Salmonella typhimurium via the oligopeptide permease. J Bacteriol. 1980 May;142(2):474–479. doi: 10.1128/jb.142.2.474-479.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]