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. 1980 Jun;142(3):819–828. doi: 10.1128/jb.142.3.819-828.1980

Dissociation of tsl-tif-Induced Filamentation and recA Protein Synthesis in Escherichia coli K-12

Olivier Huisman 1, Richard D'Ari 1, Jacqueline George 1,†
PMCID: PMC294105  PMID: 6445897

Abstract

In Escherichia coli, expression of the tif-1 mutation (in the recA gene) induces the “SOS response” at 40°C, including massive synthesis of the recA(tif) protein, cell filamentation, appearance of new repair and mutagenic activities, and prophage induction. Expression of the tsl-1 mutation (in the lexA gene) induces massive synthesis of the recA protein and cell filamentation at 42°C, although other SOS functions are not induced. In this paper we show that the septation inhibition induced in tif and tsl strains at 42°C is not due to the presence of a high concentration of recA protein since (i) no recA mutants (≤10−8) were isolated among thermoresistant nonfilamenting revertants of a tif-1 tsl-1 strain, (ii) in a tsl-1 zab-53 strain, only the low basal level of recA protein was synthesized at 42°C, yet cell division was inhibited, and (iii) in a tsl-1 recA99 (amber) strain, no recA protein could be detected at 42°C, yet cell division was inhibited. Among suppressors of tsl-tif-induced lethality are mutations at a locus which we call infB, located in the 66- to 83-min region. The infB1 mutation confers a highly pleiotropic phenotype, which is suggestive of a regulatory defect; it suppressed tsl-tif-induced filamentation but not recA protein synthesis, it did not suppress ultraviolet-induced filamentation (in a lon derivative), and it reduced but did not abolish tif-mediated induction of λ prophage and bacterial mutagenesis. The dissociation of tsl-tif-induced septation inhibition and recA protein synthesis in the tif-1 tsl-1 infB1 strain suggests that the control of SOS filamentation may not be strictly identical to the control of recA protein synthesis.

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Selected References

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