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. 1980 Jun;142(3):1036–1039. doi: 10.1128/jb.142.3.1036-1039.1980

A mutant Ebg enzyme that converts lactose into an inducer of the lac operon.

S J Rolseth, V A Fried, B G Hall
PMCID: PMC294136  PMID: 6769907

Abstract

Lactose is not itself an inducer of the lac operon, nor is it converted to an inducer by ebg+ beta-galactosidase of Escherichia coli. We report here the isolation of a mutant Ebg beta-galactosidase which is capable of converting lactose into an inducer of the lac operon.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALPERS D. H., APPEL S. H., TOMKINS G. M. A SPECTROPHOTOMETRIC ASSAY FOR THIOGALACTOSIDE TRANSACETYLASE. J Biol Chem. 1965 Jan;240:10–13. [PubMed] [Google Scholar]
  2. BURSTEIN C., COHN M., KEPES A., MONOD J. R OLE DU LACTOSE ET DE SES PRODUITS M'ETABOLIQUES DANS L'INDUCTION DE L'OP'ERON LACTOSE CHEZ ESCHERICHIA COLI. Biochim Biophys Acta. 1965 Apr 19;95:634–639. [PubMed] [Google Scholar]
  3. Gall B. G., Hartl D. L. Regulation of newly evolved enzymes. II. The ebg repressor. Genetics. 1975 Nov;81(3):427–435. doi: 10.1093/genetics/81.3.427. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hall B. G., Clarke N. D. Regulation of newly evolved enzymes. III Evolution of the ebg repressor during selection for enhanced lactase activity. Genetics. 1977 Feb;85(2):193–201. doi: 10.1093/genetics/85.2.193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hall B. G. Experimental evolution of a new enzymatic function. Kinetic analysis of the ancestral (ebg) and evolved (ebg) enzymes. J Mol Biol. 1976 Oct 15;107(1):71–84. doi: 10.1016/s0022-2836(76)80018-6. [DOI] [PubMed] [Google Scholar]
  6. Hall B. G., Hartl D. L. Regulation of newly evolved enzymes. I. Selection of a novel lactase regulated by lactose in Escherichia coli. Genetics. 1974 Mar;76(3):391–400. doi: 10.1093/genetics/76.3.391. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hall B. G. Methyl galactosidase activity: an alternative evolutionary destination for the ebgA0 gene. J Bacteriol. 1976 Apr;126(1):536–538. doi: 10.1128/jb.126.1.536-538.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hobson A. C. A mutation allowing utilisation of lactose by Escherichia coli lacY mutants defective in lactose permease. Mol Gen Genet. 1978 Apr 25;161(1):109–110. doi: 10.1007/BF00266621. [DOI] [PubMed] [Google Scholar]
  9. Huber R. E., Kurz G., Wallenfels K. A quantitation of the factors which affect the hydrolase and transgalactosylase activities of beta-galactosidase (E. coli) on lactose. Biochemistry. 1976 May 4;15(9):1994–2001. doi: 10.1021/bi00654a029. [DOI] [PubMed] [Google Scholar]
  10. Jobe A., Bourgeois S. lac Repressor-operator interaction. VI. The natural inducer of the lac operon. J Mol Biol. 1972 Aug 28;69(3):397–408. doi: 10.1016/0022-2836(72)90253-7. [DOI] [PubMed] [Google Scholar]
  11. Messer A. Lactose permeation via the arabinose transport system in Escherichia coli K-12. J Bacteriol. 1974 Oct;120(1):266–272. doi: 10.1128/jb.120.1.266-272.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rotman B., Ganesan A. K., Guzman R. Transport systems for galactose and galactosides in Escherichia coli. II. Substrate and inducer specificities. J Mol Biol. 1968 Sep 14;36(2):247–260. doi: 10.1016/0022-2836(68)90379-3. [DOI] [PubMed] [Google Scholar]

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