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Binding of BlaI to dsDNA. Dissociation constants obtained from the fit of the anisotropy data to the model presented in Scheme 1. The anisotropy data were fit using equation E1, shown in Supporting Information.
| Kd1 (µM) | Kd2 (µM) | Kd3 (µM) | |
|---|---|---|---|
| R1 dyad | 0.8 ± 0.1 | 20 ± 1 | 10 ± 2 |
| Z dyad | 0.05 ± 0.04 | 10.7 ± 0.3 | 0.3 ± 0.3 |
| R1-Z dyads | 0.45 ± 0.07 | 2.6 ± 0.1 | 0.72 ± 0.07 |
