Table 1.
Characterization of isolated dendrimer with precise numbers of ligands.
| HPLC Analysis | NMR Analysis | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Nominal no. of li- gands per dendrimer |
Mass re- covered [mg] |
No. li- gands per dendrimer |
Purity [%][a]Increase in purity[b] |
No. li- gands per dendrimer |
Difference [%][c] |
No. li- gands per dendrimer |
Difference [%][c] |
||
| 0 | 10.4 | 0.0 | 100 | 14× | 0 | 0 | 0 | 0 | Method |
| 1 | 10.1 | 1.0 | 97 | 6× | 1.2 | 20 | 1.1 | 10 | 1Method |
| 2 | 8.5 | 1.9 | 93 | 6× | 2.4 | 26 | 2.2 | 14 | 2 |
| 3 | 9.5 | 2.7 | 88 | 7× | 3.4 | 26 | 3.2 | 17 | |
| 4 | 8.7 | 3.8 | 86 | 9× | 4.3 | 13 | 3.9 | 3 | |
| 5 | 5.4 | 4.8 | 84 | 10× | 4.9 | 2 | 4.5 | 7 | |
| 6 | 6.7 | 5.8 | 84 | 12× | 5.7 | 2 | 5.2 | 10 | |
| 7 | 1.5 | 6.7 | 82 | 14× | 6.8 | 3 | 5.8 | 13 | |
| 8 | 1.8 | 7.7 | 79 | 16× | 7.8 | 1 | 6.9 | 10 | |
[a]
Defined by the number of ligands per dendrimer.
[b]
Fold increase in purity is relative to the amount of the component in the distribution (dendrimer 3).
[c]
Difference is calculated by the NMR ratio minus the HPLC ratio, divided by the HPLC ratio.