Table 1.
HPLC Analysis | NMR Analysis | ||||||||
---|---|---|---|---|---|---|---|---|---|
Nominal no. of li- gands per dendrimer |
Mass re- covered [mg] |
No. li- gands per dendrimer |
Purity [%][a]Increase in purity[b] |
No. li- gands per dendrimer |
Difference [%][c] |
No. li- gands per dendrimer |
Difference [%][c] |
||
0 | 10.4 | 0.0 | 100 | 14× | 0 | 0 | 0 | 0 | Method |
1 | 10.1 | 1.0 | 97 | 6× | 1.2 | 20 | 1.1 | 10 | 1Method |
2 | 8.5 | 1.9 | 93 | 6× | 2.4 | 26 | 2.2 | 14 | 2 |
3 | 9.5 | 2.7 | 88 | 7× | 3.4 | 26 | 3.2 | 17 | |
4 | 8.7 | 3.8 | 86 | 9× | 4.3 | 13 | 3.9 | 3 | |
5 | 5.4 | 4.8 | 84 | 10× | 4.9 | 2 | 4.5 | 7 | |
6 | 6.7 | 5.8 | 84 | 12× | 5.7 | 2 | 5.2 | 10 | |
7 | 1.5 | 6.7 | 82 | 14× | 6.8 | 3 | 5.8 | 13 | |
8 | 1.8 | 7.7 | 79 | 16× | 7.8 | 1 | 6.9 | 10 |
Defined by the number of ligands per dendrimer.
Fold increase in purity is relative to the amount of the component in the distribution (dendrimer 3).
Difference is calculated by the NMR ratio minus the HPLC ratio, divided by the HPLC ratio.