Abstract
Short-chain acid-soluble polyphosphates were extracted from growing cultures of Saccharomyces cerevisiae, and the changes in the levels of these compounds were determined. The production of acid-soluble polyphosphates correlated with the mitochondrial activities since it occurred in two bursts in respiration-competent yeast cells and in only one burst in respiration-deficient yeast cells. The possible role of these compounds is discussed.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cashel M., Lazzarini R. A., Kalbacher B. An improved method for thin-layer chromatography of nucleotide mixtures containing 32P-labelled orthophosphate. J Chromatogr. 1969 Mar 11;40(1):103–109. doi: 10.1016/s0021-9673(01)96624-5. [DOI] [PubMed] [Google Scholar]
- Fiechter A. Continuous cultivation of yeasts. Methods Cell Biol. 1975;11:97–130. doi: 10.1016/s0091-679x(08)60319-2. [DOI] [PubMed] [Google Scholar]
- KATCHMAN B. J., FETTY W. O. Phosphorus metabolism in growing cultures of Saccharomyces cerevisiae. J Bacteriol. 1955 Jun;69(6):607–615. doi: 10.1128/jb.69.6.607-615.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kowalski S., Fresco J. R. Preparation of highly labeled (32P)nucleic acids from yeasts; isolation of "denaturable" leucine acceptor transfer RNA. Science. 1971 Apr 23;172(3981):384–385. doi: 10.1126/science.172.3981.384. [DOI] [PubMed] [Google Scholar]
- Ludwig J. R., 2nd, Oliver S. G., McLaughlin C. S. The effect of amino acids on growth and phosphate metabolism in a prototrophic yeast strain. Biochem Biophys Res Commun. 1977 Nov 7;79(1):16–23. doi: 10.1016/0006-291x(77)90054-7. [DOI] [PubMed] [Google Scholar]
- Rhaese H. J., Scheckel R., Groscurth R., Stamminger G. Studies on the control of development. Highly phosphorylated nucleotides (HPN) are correlated with ascospore formation in Saccharomyces cerevisiae. Mol Gen Genet. 1979 Feb 16;170(1):57–65. doi: 10.1007/BF00268580. [DOI] [PubMed] [Google Scholar]