Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1980 Aug;143(2):921–925. doi: 10.1128/jb.143.2.921-925.1980

Use of gene cloning to determine polarity of an operon: genes carAB of Escherichia coli.

M Crabeel, D Charlier, G Weyens, A Feller, A Piérard, N Glansdorff
PMCID: PMC294395  PMID: 6451616

Abstract

A gene-cloning approach was used to determine the transcription polarity of the carbamoylphosphate operon (carAB) of Escherichia coli. In agreement with the accompanying paper (J. Bacteriol. 143:914-920, 1980), our results lead to the conclusion that carA is the proximal gene of the carAB operon.

Full text

PDF
921

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann B. J., Low K. B., Taylor A. L. Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976 Mar;40(1):116–167. doi: 10.1128/br.40.1.116-167.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Court D., Sato K. Studies of novel transducing variants of lambda: dispensability of genes N and Q. Virology. 1969 Oct;39(2):348–352. doi: 10.1016/0042-6822(69)90060-9. [DOI] [PubMed] [Google Scholar]
  3. Crabeel M., Charlier D., Glansdorff N. Studies on the bipolar argECBH operon of E. coli: characterization of restriction endonuclease fragments obtained from gammadargECBH transducing phages and a ColE1 argECBH plasmid. Mol Gen Genet. 1977 Mar 7;151(2):161–168. doi: 10.1007/BF00338690. [DOI] [PubMed] [Google Scholar]
  4. Crabeel M., Charlier D., Weyens G., Feller A., Glansdorff N., Piérard A. Repressible expression of an Escherichia coli carA gene inserted into a colicinogenic factor [proceedings]. Arch Int Physiol Biochim. 1978 Oct;86(4):912–913. [PubMed] [Google Scholar]
  5. Gigot D., Crabeel M., Feller A., Charlier D., Lissens W., Glansdorff N., Piérard A. Patterns of polarity in the Escherichia coli car AB gene cluster. J Bacteriol. 1980 Aug;143(2):914–920. doi: 10.1128/jb.143.2.914-920.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Glansdorff N., Dambly C., Palchaudhuri S., Crabeel M., Piérard A., Halleux P. Isolation and heteroduplex mapping of a lambda transducing bacteriophage carrying the structural genes for carbamoylphosphate synthase: regulation of enzyme synthesis in Escherichia coli K-12 lysogens. J Bacteriol. 1976 Jul;127(1):302–308. doi: 10.1128/jb.127.1.302-308.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lissens W., Cunin R., Kelker N., Glansdorff N., Piérard A. In vitro synthesis of Escherichia coli carbamoylphosphate synthase: evidence for participation of the arginine repressor in cumulative repression. J Bacteriol. 1980 Jan;141(1):58–66. doi: 10.1128/jb.141.1.58-66.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mazaitis A. J., Palchaudhuri S., Glansdorff N., Maas W. K. Isolation and characterization of lambdadargECBH transducing phages and heteroduplex analysis of the argECBH cluster. Mol Gen Genet. 1976 Jan 16;143(2):185–196. doi: 10.1007/BF00266921. [DOI] [PubMed] [Google Scholar]
  9. Mergeay M., Gigot D., Beckmann J., Glansdorff N., Piérard A. Physiology and genetics of carbamoylphosphate synthesis in Escherichia coli K12. Mol Gen Genet. 1974;133(4):299–316. doi: 10.1007/BF00332706. [DOI] [PubMed] [Google Scholar]
  10. Piérard A., Glansdorff N., Gigot D., Crabeel M., Halleux P., Thiry L. Repression of Escherichia coli carbamoylphosphate synthase: relationships with enzyme synthesis in the arginine and pyrimidine pathways. J Bacteriol. 1976 Jul;127(1):291–301. doi: 10.1128/jb.127.1.291-301.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Piérard A., Glansdorff N., Mergeay M., Wiame J. M. Control of the biosynthesis of carbamoyl phosphate in Escherichia coli. J Mol Biol. 1965 Nov;14(1):23–36. doi: 10.1016/s0022-2836(65)80226-1. [DOI] [PubMed] [Google Scholar]
  12. Piérard A., Glansdorff N., Yashphe J. Mutations affecting uridine monophosphate pyrophosphorylase or the argR gene in Escherichia coli. Effects on carbamoyl phosphate and pyrimidine biosynthesis and on uracil uptake. Mol Gen Genet. 1972;118(3):235–245. doi: 10.1007/BF00333460. [DOI] [PubMed] [Google Scholar]
  13. Piérard A., Wiame J. M. Regulation and mutation affecting a glutamine dependent formation of carbamyl phosphate in Escherichia coli. Biochem Biophys Res Commun. 1964 Feb 18;15(1):76–81. doi: 10.1016/0006-291x(64)90106-8. [DOI] [PubMed] [Google Scholar]
  14. Schrenk W. J., Weisberg R. A. A simple method for making new transducing lines of coliphage lambda. Mol Gen Genet. 1975;137(2):101–107. doi: 10.1007/BF00341676. [DOI] [PubMed] [Google Scholar]
  15. Thomas M., Davis R. W. Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease. J Mol Biol. 1975 Jan 25;91(3):315–328. doi: 10.1016/0022-2836(75)90383-6. [DOI] [PubMed] [Google Scholar]
  16. Trotta P. P., Pinkus L. M., Haschemeyer R. H., Meister A. Reversible dissociation of the monomer of glutamine-dependent carbamyl phosphate synthetase into catalytically active heavy and light subunits. J Biol Chem. 1974 Jan 25;249(2):492–499. [PubMed] [Google Scholar]
  17. Womble D. D., Taylor D. P., Rownd R. H. Method for obtaining more-accurate covalently closed circular plasmid-to-chromosome ratios from bacterial lysates by dye-buoyant density centrifugation. J Bacteriol. 1977 Apr;130(1):148–153. doi: 10.1128/jb.130.1.148-153.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES