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. 1980 Sep;143(3):1265–1274. doi: 10.1128/jb.143.3.1265-1274.1980

Kinetics and regulation of cell-free alkaline phosphatase synthesis.

C Pratt
PMCID: PMC294493  PMID: 6157671

Abstract

Regulation of alkaline phosphatase (EC 3.1.3.1) synthesis in a cell-free system from Escherichia coli has been observed. Synthesis from transducing phage deoxyribonucleic acid templates carrying phoA+ occurred in S30 fractions from wild-type or alkaline phosphatase-constitutive mutants. It did not occur in S30) fractions from alkaline phosphatase-negative mutants (phoB). The hybrid gene phoA-lacZ was also subject to phoB control, implying that phoA transcription is regulated. The yield of active alkaline phosphatase per phoA+ gene copy from cell-free synthesis was similar to that of beta-galactosidase. Alkaline phosphatase activity took longer to appear than beta-galactosidase activity. Synthesis of alkaline phosphatase subunits was not delayed, suggesting that a minimum number of subunits are synthesized before formation of active alkaline phosphatase occurs.

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Selected References

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