Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1980 Sep;143(3):1289–1294. doi: 10.1128/jb.143.3.1289-1294.1980

Genetic characterization of a filament-forming, lipoprotein-deficient mutant of Escherichia coli.

S Torti, J T Park
PMCID: PMC294499  PMID: 6997268

Abstract

The fam-715 allele of Escherichia coli ST715, previously described as a temperature-sensitive filament former with reduced levels of lipoprotein at the nonpermissive temperature (S. V. Torti and J. T. Park, Nature [London] 263: 323--326, 1976), was mapped at 74 min. This mutation appears to be amber. It is recessive and can be complemented by F' plasmids carrying the wild-type allele or by an F' plasmid carrying an amber suppressor. Isotopic labeling experiments as well as map position differentiate the fam-715 allele from lipoprotein structural gene mutations.

Full text

PDF
1289

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann B. J., Low K. B., Taylor A. L. Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976 Mar;40(1):116–167. doi: 10.1128/br.40.1.116-167.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Braun V. Covalent lipoprotein from the outer membrane of Escherichia coli. Biochim Biophys Acta. 1975 Oct 31;415(3):335–377. doi: 10.1016/0304-4157(75)90013-1. [DOI] [PubMed] [Google Scholar]
  3. Fung J., MacAlister T. J., Rothfield L. I. Role of murein lipoprotein in morphogenesis of the bacterial division septum: phenotypic similarity of lkyD and lpo mutants. J Bacteriol. 1978 Mar;133(3):1467–1471. doi: 10.1128/jb.133.3.1467-1471.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hirota Y., Suzuki H., Nishimura Y., Yasuda S. On the process of cellular division in Escherichia coli: a mutant of E. coli lacking a murein-lipoprotein. Proc Natl Acad Sci U S A. 1977 Apr;74(4):1417–1420. doi: 10.1073/pnas.74.4.1417. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Inouye S., Lee N., Inouye M., Wu H. C., Suzuki H., Nishimura Y., Iketani H., Hirota Y. Amino acid replacement in a mutant lipoprotein of the Escherichia coli outer membrane. J Bacteriol. 1977 Oct;132(1):308–313. doi: 10.1128/jb.132.1.308-313.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Inukai M., Takeuchi M., Shimizu K., Arai M. Mechanism of action of globomycin. J Antibiot (Tokyo) 1978 Nov;31(11):1203–1205. doi: 10.7164/antibiotics.31.1203. [DOI] [PubMed] [Google Scholar]
  7. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  8. Nakamura K., Pirtle R. M., Inouye M. Homology of the gene coding for outer membrane lipoprotein within various Gram-negative bacteria. J Bacteriol. 1979 Jan;137(1):595–604. doi: 10.1128/jb.137.1.595-604.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ricard M., Hirota Y. Process of cellular division in Escherichia coli: physiological study on thermosensitive mutants defective in cell division. J Bacteriol. 1973 Oct;116(1):314–322. doi: 10.1128/jb.116.1.314-322.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sato T., Yura T. Chromosomal location and expression of the structural gene for major outer membrane protein Ia of Escherichia coli K-12 and of the homologous gene of Salmonella typhimurium. J Bacteriol. 1979 Aug;139(2):468–477. doi: 10.1128/jb.139.2.468-477.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sturgeon J. A., Ingram L. O. Low-temperature conditional cell division mutants of Escherichia coli. J Bacteriol. 1978 Jan;133(1):256–264. doi: 10.1128/jb.133.1.256-264.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Torti S. V., Park J. T. Lipoprotein of gram-negative bacteria is essential for growth and division. Nature. 1976 Sep 23;263(5575):323–326. doi: 10.1038/263323a0. [DOI] [PubMed] [Google Scholar]
  13. VOGEL H. J., BONNER D. M. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed] [Google Scholar]
  14. Wanner B. L., Sarthy A., Beckwith J. Escherichia coli pleiotropic mutant that reduces amounts of several periplasmic and outer membrane proteins. J Bacteriol. 1979 Oct;140(1):229–239. doi: 10.1128/jb.140.1.229-239.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Weigand R. A., Vinci K. D., Rothfield L. I. Morphogenesis of the bacterial division septum: a new class of septation-defective mutants. Proc Natl Acad Sci U S A. 1976 Jun;73(6):1882–1886. doi: 10.1073/pnas.73.6.1882. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Willsky G. R., Bennett R. L., Malamy M. H. Inorganic phosphate transport in Escherichia coli: involvement of two genes which play a role in alkaline phosphatase regulation. J Bacteriol. 1973 Feb;113(2):529–539. doi: 10.1128/jb.113.2.529-539.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wu H. C., Hou C., Lin J. J., Yem D. W. Biochemical characterization of a mutant lipoprotein of Escherichia coli. Proc Natl Acad Sci U S A. 1977 Apr;74(4):1388–1392. doi: 10.1073/pnas.74.4.1388. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wu H. C., Lin J. J. Escherichia coli mutants altered in murein lipoprotein. J Bacteriol. 1976 Apr;126(1):147–156. doi: 10.1128/jb.126.1.147-156.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Yem D. W., Wu H. C. Physiological characterization of an Escherichia coli mutant altered in the structure of murein lipoprotein. J Bacteriol. 1978 Mar;133(3):1419–1426. doi: 10.1128/jb.133.3.1419-1426.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES