Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1980 Sep;143(3):1403–1410. doi: 10.1128/jb.143.3.1403-1410.1980

Proline: an essential intermediate in arginine degradation in Saccharomyces cerevisiae.

M C Brandriss, B Magasanik
PMCID: PMC294521  PMID: 6997271

Abstract

Results of studies on proline-nonutilizing (Put-) mutants of the yeast Saccharomyces cerevisiae indicate that proline is an essential intermediate in the degradation of arginine. Put- mutants excreted proline when grown on arginine or ornithine as the sole nitrogen source. Yeast cells contained a single enzyme, delta 1-pyrroline-5-carboxylate (P5C) dehydrogenase, which is essential for the complete degradation of both proline and arginine. The sole inducer of this enzyme was found to be proline. P5C dehydrogenase converted P5C to glutamate, but only when the P5C was derived directly from proline. When the P5C was derived from ornithine, it was first converted to proline by the enzyme P5C reductase. Proline was then converted back to P5C and finally to glutamate by the Put enzymes proline oxidase and P5C dehydrogenase.

Full text

PDF
1403

Images in this article

1408Image
on p.1408

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bechet J., Wiame J. M. Indication of a specific regulatory binding protein for ornithinetranscarbamylase in Saccharomyces cerevisiae. Biochem Biophys Res Commun. 1965 Nov 8;21(3):226–234. doi: 10.1016/0006-291x(65)90276-7. [DOI] [PubMed] [Google Scholar]
  2. Brandriss M. C. Isolation and preliminary characterization of Saccharomyces cerevisiae proline auxotrophs. J Bacteriol. 1979 Jun;138(3):816–822. doi: 10.1128/jb.138.3.816-822.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brandriss M. C., Magasanik B. Genetics and physiology of proline utilization in Saccharomyces cerevisiae: enzyme induction by proline. J Bacteriol. 1979 Nov;140(2):498–503. doi: 10.1128/jb.140.2.498-503.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brandriss M. C., Magasanik B. Genetics and physiology of proline utilization in Saccharomyces cerevisiae: mutation causing constitutive enzyme expression. J Bacteriol. 1979 Nov;140(2):504–507. doi: 10.1128/jb.140.2.504-507.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Deschamps J., Wiame J. M. Mating-type effect on cis mutations leading to constitutivity of ornithine transaminase in diploid cells of Saccharomyces cerevisiae. Genetics. 1979 Jul;92(3):749–758. doi: 10.1093/genetics/92.3.749. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jauniaux J. C., Urrestarazu L. A., Wiame J. M. Arginine metabolism in Saccharomyces cerevisiae: subcellular localization of the enzymes. J Bacteriol. 1978 Mar;133(3):1096–1107. doi: 10.1128/jb.133.3.1096-1107.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. MIDDELHOVEN W. J. THE PATHWAY OF ARGININE BREAKDOWN IN SACCHAROMYCES CEREVISIAE. Biochim Biophys Acta. 1964 Dec 9;93:650–652. doi: 10.1016/0304-4165(64)90349-6. [DOI] [PubMed] [Google Scholar]
  9. Mestichelli L. J., Gupta R. N., Spenser I. D. The biosynthetic route from ornithine to proline. J Biol Chem. 1979 Feb 10;254(3):640–647. [PubMed] [Google Scholar]
  10. Middelhoven W. J. The derepression of arginase and of ornithine transaminase in nitrogen-starved baker's yeast. Biochim Biophys Acta. 1968 Mar 11;156(2):440–443. doi: 10.1016/0304-4165(68)90284-5. [DOI] [PubMed] [Google Scholar]
  11. Stewart P. R. Analytical methods for yeasts. Methods Cell Biol. 1975;12:111–147. doi: 10.1016/s0091-679x(08)60955-3. [DOI] [PubMed] [Google Scholar]
  12. Voellmy R., Leisinger T. Dual role for N-2-acetylornithine 5-aminotransferase from Pseudomonas aeruginosa in arginine biosynthesis and arginine catabolism. J Bacteriol. 1975 Jun;122(3):799–809. doi: 10.1128/jb.122.3.799-809.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Whitney P. A., Magasanik B. The induction of arginase in Saccharomyces cerevisiae. J Biol Chem. 1973 Sep 10;248(17):6197–6202. [PubMed] [Google Scholar]
  14. Williams I., Frank L. Improved chemical synthesis and enzymatic assay of delta-1-pyrroline-5-carboxylic acid. Anal Biochem. 1975 Mar;64(1):85–97. doi: 10.1016/0003-2697(75)90408-x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES