Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1980 Sep;143(3):1444–1449. doi: 10.1128/jb.143.3.1444-1449.1980

Generalized transduction in the enterobacterial phytopathogen Erwinia chrysanthemi.

A K Chatterjee, M A Brown
PMCID: PMC294530  PMID: 6931829

Abstract

Bacteriophages induced by mitomycin treatment of Erwinia chrysanthemi KS612 produced plaques on lawns of E. chrysanthemi EC183 and KS605. Bacteriophage Erch-12, purified from one such plaque, transferred an array of chromosomal genes (arg, leu, his, ser, thr, trp, ura) to appropriate recipient strains derived from E. chrysanthemi EC 183. Recombinants were formed in the absence of cellular contact between donor and recipient bacteria and in the presence of deoxyribonuclease. Ultraviolet irradiation of the bacteriophage stimulated transductional frequency. Linkage was detected in two-factor crosses between the loci thr and ser and between rif and ade; several closely linked mutations in ser were mapped with respect to thr.

Full text

PDF
1444

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BENZINGER R., HARTMAN P. E. Effects of ultraviolet light on transducing phage P22. Virology. 1962 Dec;18:614–626. doi: 10.1016/0042-6822(62)90064-8. [DOI] [PubMed] [Google Scholar]
  2. Bachmann B. J., Low K. B. Linkage map of Escherichia coli K-12, edition 6. Microbiol Rev. 1980 Mar;44(1):1–56. doi: 10.1128/mr.44.1.1-56.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Campos J. M., Geisselsoder J., Zusman D. R. Isolation of bacteriophage MX4, a generalized transducing phage for Myxococcus xanthus. J Mol Biol. 1978 Feb 25;119(2):167–178. doi: 10.1016/0022-2836(78)90431-x. [DOI] [PubMed] [Google Scholar]
  4. Chatterjee A. K. Acceptance by Erwinia spp. of R plasmid R68.45 and its ability to mobilize the chromosome of Erwinia chrysanthemi. J Bacteriol. 1980 Apr;142(1):111–119. doi: 10.1128/jb.142.1.111-119.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chatterjee A. K., Starr M. P. Donor strains of the soft-rot bacterium Erwinia chrysanthemi and conjugational transfer of the pectolytic capacity. J Bacteriol. 1977 Dec;132(3):862–869. doi: 10.1128/jb.132.3.862-869.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chatterjee A. K., Starr M. P. Gene transmission among strains of Erwinia amylovora. J Bacteriol. 1973 Dec;116(3):1100–1106. doi: 10.1128/jb.116.3.1100-1106.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Drexler H. Transduction by bacteriophage T1. Proc Natl Acad Sci U S A. 1970 Aug;66(4):1083–1088. doi: 10.1073/pnas.66.4.1083. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ely B., Johnson R. C. Generalized Transduction in CAULOBACTER CRESCENTUS. Genetics. 1977 Nov;87(3):391–399. doi: 10.1093/genetics/87.3.391. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Goldberg R. B., Bender R. A., Streicher S. L. Direct selection for P1-sensitive mutants of enteric bacteria. J Bacteriol. 1974 Jun;118(3):810–814. doi: 10.1128/jb.118.3.810-814.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Guerola N., Ingraham J. L., Cerdá-Olmedo E. Induction of closely linked multiple mutations by nitrosoguanidine. Nat New Biol. 1971 Mar 24;230(12):122–125. doi: 10.1038/newbio230122a0. [DOI] [PubMed] [Google Scholar]
  11. Henner D. J., Hoch J. A. The Bacillus subtilis chromosome. Microbiol Rev. 1980 Mar;44(1):57–82. doi: 10.1128/mr.44.1.57-82.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Herman N. J., Juni E. Isolation and characterization of a generalized transducing bacteriophage for Acinetobacter. J Virol. 1974 Jan;13(1):46–52. doi: 10.1128/jvi.13.1.46-52.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mekalanos J. J., Sublett R. D., Romig W. R. Genetic mapping of toxin regulatory mutations in Vibrio cholerae. J Bacteriol. 1979 Sep;139(3):859–865. doi: 10.1128/jb.139.3.859-865.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Novick R. P., Clowes R. C., Cohen S. N., Curtiss R., 3rd, Datta N., Falkow S. Uniform nomenclature for bacterial plasmids: a proposal. Bacteriol Rev. 1976 Mar;40(1):168–189. doi: 10.1128/br.40.1.168-189.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sanderson K. E., Hartman P. E. Linkage map of Salmonella typhimurium, edition V. Microbiol Rev. 1978 Jun;42(2):471–519. doi: 10.1128/mr.42.2.471-519.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Starr M. P., Chatterjee A. K. The genus Erwinia: enterobacteria pathogenic to plants and animals. Annu Rev Microbiol. 1972;26:389–426. doi: 10.1146/annurev.mi.26.100172.002133. [DOI] [PubMed] [Google Scholar]
  17. WILSON D. E. The effects of ultraviolet light and ionizing radiation on the transduction of Escherichia coli by phage P1. Virology. 1960 Jul;11:533–546. doi: 10.1016/0042-6822(60)90098-2. [DOI] [PubMed] [Google Scholar]
  18. Watson J. M., Holloway B. W. Linkage map of Pseudomonas aeruginosa PAT. J Bacteriol. 1978 Nov;136(2):507–521. doi: 10.1128/jb.136.2.507-521.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES