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Three different malignant T-cell lines (A–C, SeAx, JB6 and MyLa respectively) were transiently transfected with non-targeting (NT), PDCD10- or STAT3-specific siRNA. Western blot of whole-cell extracts were prepared from the cells 48 hours after transfection and probed with antibodies against PDCD10, STAT3 and actin.
