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. 1980 Sep;143(3):1509–1512. doi: 10.1128/jb.143.3.1509-1512.1980

Substrate channeling: alpha-ketobutyrate inhibition of acetohydroxy acid synthase in Salmonella typhimurium.

K J Shaw, C M Berg
PMCID: PMC294547  PMID: 6997277

Abstract

Excess alpha-ketobutyrate inhibited the growth of Salmonella typhimurium LT2 by inhibiting the acetohydroxy acid synthase-catalyzed synthesis of alpha-acetolactate (a valine precursor). As a result, cells were starved for valine, and both ilvB (encoding acetohydroxy acid synthase I) and ilvGEDA (ilvG encodes acetohydroxy acid synthase II) were derepressed. The addition of valine reversed the effects of alpha-ketobutyrate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berg C. M., Shaw K. J., Vender J., Borucka-Mankiewicz M. Physiological characterization of polar Tn5-induced isoleucine-valine auxotrophs in Escherichia coli K.12: evidence for an internal promoter in the ilvOGEDA operon. Genetics. 1979 Oct;93(2):308–319. [PMC free article] [PubMed] [Google Scholar]
  2. Blatt J. M., Pledger W. J., Umbarger H. E. Isoleucine and valine metabolism in Escherichia coli. XX. Multiple forms of acetohydroxy acid synthetase. Biochem Biophys Res Commun. 1972 Jul 25;48(2):444–450. doi: 10.1016/s0006-291x(72)80071-8. [DOI] [PubMed] [Google Scholar]
  3. Cioffi E. A., Shaw K. J., Bailey W. F., Berg C. M. Improved synthesis of the sodium salt of DL-alpha, beta-dihydroxyisovaleric acid. Anal Biochem. 1980 May 15;104(2):485–488. doi: 10.1016/0003-2697(80)90104-9. [DOI] [PubMed] [Google Scholar]
  4. Kleckner N., Chan R. K., Tye B. K., Botstein D. Mutagenesis by insertion of a drug-resistance element carrying an inverted repetition. J Mol Biol. 1975 Oct 5;97(4):561–575. doi: 10.1016/s0022-2836(75)80059-3. [DOI] [PubMed] [Google Scholar]
  5. LEAVITT R. I., UMBARGER H. E. Isoleucine and valine metabolism in Escherichia coli. X. The enzymatic formation of acetohydroxybutyrate. J Biol Chem. 1961 Sep;236:2486–2491. [PubMed] [Google Scholar]
  6. O'Neill J. P., Freundlich M. Two forms of biosynthetic acetohydroxy acid synthetase in Salmonella typhimurium. Biochem Biophys Res Commun. 1972 Jul 25;48(2):437–443. doi: 10.1016/s0006-291x(72)80070-6. [DOI] [PubMed] [Google Scholar]
  7. Shaw K. J., Berg C. M., Sobol T. J. Salmonella typhimurium mutants defective in acetohydroxy acid synthases I and II. J Bacteriol. 1980 Mar;141(3):1258–1263. doi: 10.1128/jb.141.3.1258-1263.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Smith J. M., Smith F. J., Umbarger H. E. Mutations affecting the formation of acetohydroxy acid synthase II in Escherichia coli K-12. Mol Gen Genet. 1979 Feb 1;169(3):299–314. doi: 10.1007/BF00382276. [DOI] [PubMed] [Google Scholar]

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