Figure 4.

qPCR analysis of genes related to cumulus expansion in WT (white bars) and Inha^−/− (black bars) preovulatory granulosa cells collected from mice treated with and without hCG. Preovulatory granulosa cells were collected 44–46 h after PMSG injection without (−) or with hCG for 6 h. qPCR results are expressed as RQ. Has2 (A), Areg (C), Ereg (D), Ptgs2 (E), and Ptx3 (F) are more highly up-regulated in hCG-treated Inha^−/− preovulatory granulosa cells compared with hCG-treated WT cells, whereas Tnfaip6 (B) shows a similar fold induction. Data are mean ± sem of a minimum of three independent samples with the control mean set to 1. Different letters above the bars indicate statistical significance at P < 0.05 by one-way ANOVA and followed by Fisher LSD post hoc tests.