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. 1994 Nov;94(5):1968–1973. doi: 10.1172/JCI117548

Role of the GRO family of chemokines in monocyte adhesion to MM-LDL-stimulated endothelium.

D Schwartz 1, A Andalibi 1, L Chaverri-Almada 1, J A Berliner 1, T Kirchgessner 1, Z T Fang 1, P Tekamp-Olson 1, A J Lusis 1, C Gallegos 1, A M Fogelman 1, et al.
PMCID: PMC294616  PMID: 7962543

Abstract

We have previously shown that treatment of endothelial cells with minimally modified LDL (MM-LDL) induces the binding of monocytes to unknown endothelial receptor molecules. We now report that a member of the GRO family of chemokines plays a role in MM-LDL-induced monocyte binding. A cDNA library made from rabbit aortic endothelial cells (RAEC) treated with MM-LDL was expression screened for molecules inducing binding of a human monocyte cell line (THP-1). A cDNA was isolated with 75% homology to GRO. GRO mRNA levels were significantly elevated after exposure of RAEC or human aortic endothelial cells (HAEC) to MM-LDL. HAEC treated with MM-LDL displayed an increase in a surface-associated protein that bound to antibody against GRO despite low levels of GRO in the medium. Antibody to GRO significantly inhibited the binding of monocytes to MM-LDL-treated RAEC and HAEC. The increase in GRO expression and monocyte binding were reduced by incubating MM-LDL-treated endothelial cells with heparin (in a method that releases heparan sulfate bound molecules from the cell surface). These results suggest that GRO related chemokines are bound to the surface of MM-LDL-treated endothelial cells and may contribute to the monocyte adhesion induced by MM-LDL.

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Selected References

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