Skip to main content
. Author manuscript; available in PMC: 2011 Nov 10.
Published in final edited form as: Virology. 2010 Sep 6;407(1):160–170. doi: 10.1016/j.virol.2010.08.009

Fig. 1.

Fig. 1

OCLN EL2 is essential to mediate HCVpp entry. (A) 5×105 786-O cells were transduced with lentiviruses to express Flag-tagged hOCLN, OCLN/ΔN, OCLN/ΔC, OCLN/ΔEL1, and OCLN/ΔEL2. pTrip-GFP virus (expressing the green fluorescent protein) was included to control for transduction. Cell lysates were made at 48 hr p.i. and immunoblotted with an anti-Flag antibody (top panel) or a monoclonal anti-OCLN antibody (bottom panel). OCLN/ΔC (indicated with a star sign in top panel) could not be recognized by the anti-OCLN antibody as this antibody was raised against the C-terminus of OCLN. (B) All of the OCLN constructs except OCLN/ΔEL2 rendered 786-O cells susceptible to HCVpp infection. Data are representative of at least three independent experiments and are presented as mean ± standard deviation.