Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1980 Oct;144(1):457–459. doi: 10.1128/jb.144.1.457-459.1980

Improved method for conjugative transfer by filter mating of Streptococcus pneumoniae.

M D Smith, W R Guild
PMCID: PMC294685  PMID: 6998966

Abstract

The frequency of conjugation during filter mating of pneumococcus was increased 10- to 100-fold when the filter was embedded in agar during incubation instead of being on the surface. The major effect was not due to protection from oxygen. The factor of increase was similar for transfer of plasmids and of chromosomal insertions of drug resistance elements.

Full text

PDF
457

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Guild W. R., Shoemaker N. B. Mismatch correction in pneumococcal transformation: donor length and hex-dependent marker efficiency. J Bacteriol. 1976 Jan;125(1):125–135. doi: 10.1128/jb.125.1.125-135.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Porter R. D., Guild W. R. Characterization of some pneumococcal bacteriophages. J Virol. 1976 Aug;19(2):659–667. doi: 10.1128/jvi.19.2.659-667.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Shoemaker N. B., Smith M. D., Guild W. R. DNase-resistant transfer of chromosomal cat and tet insertions by filter mating in Pneumococcus. Plasmid. 1980 Jan;3(1):80–87. doi: 10.1016/s0147-619x(80)90036-0. [DOI] [PubMed] [Google Scholar]
  4. Shoemaker N. B., Smith M. D., Guild W. R. Organization and transfer of heterologous chloramphenicol and tetracycline resistance genes in pneumococcus. J Bacteriol. 1979 Aug;139(2):432–441. doi: 10.1128/jb.139.2.432-441.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Smith M. D., Shoemaker N. B., Burdett V., Guild W. R. Transfer of plasmids by conjugation in Streptococcus pneumonias. Plasmid. 1980 Jan;3(1):70–79. doi: 10.1016/s0147-619x(80)90035-9. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES