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. 1980 Nov;144(2):721–731. doi: 10.1128/jb.144.2.721-731.1980

Yeast-phase cell cycle of the polymorphic fungus Wangiella dermatitidis.

R L Roberts, P J Szaniszlo
PMCID: PMC294722  PMID: 7430070

Abstract

The yeast-phase cell cycle of Wangiella dermatitidis was studied using flow microfluorimetry and the deoxyribonucleic acid (DNA) synthesis inhibitor hydroxyurea (HU). Exposure of exponential-phase yeastlike cells to 0.1 M HU for 3 to 6 h resulted in the arrest of the cells in DNA synthesis and produced a nearly homogeneous population of unbudded cells. Treatment of the yeast-phase cells with HU for 9 h or longer resulted in the accumulation of the cells predominantly as budded forms having either a single nucleus in the mother cell or a single nucleus arrested in the isthmus between the mother cell and the daughter bud. Exposure of unbudded stationary-phase cells to 0.1 M HU resulted in the accumulation of the cells in the same phenotypes. Analysis by flow microfluorimetry and cell counts of HU-inhibited mithramycin-stained cells indicated that the eventual progress of HU-inhibited cells from unbudded to the two budded forms was due to the limited continuation of the growth sequence of the cell cycle even in the absence of DNA synthesis, nuclear division, and in some cases nuclear migration. On the basis of these observations and the results of flow microfluorimetric analysis of exponential-phase cells, a map of the yeast-phase cell cycle was constructed. The cycle appears to consist of two independent sequences of events, a budding growth sequence and a DNA division sequence. The nuclear division cycle of yeast-phase cells growing exponentially with a 4.5-h generation time is composed of a G1 interval of 148 min, as S phase of 16 min, and a G2 plus M interval of 107 min.

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Selected References

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  1. Carmi P., Koltin Y., Stamberg J. Meiosis in Schizophyllum commune: premeiotic DNA replication and meiotic synchrony induced with hydroxyurea. Genet Res. 1978 Jun;31(3):215–226. doi: 10.1017/s0016672300018000. [DOI] [PubMed] [Google Scholar]
  2. Dean P. N., Jett J. H. Mathematical analysis of DNA distributions derived from flow microfluorometry. J Cell Biol. 1974 Feb;60(2):523–527. doi: 10.1083/jcb.60.2.523. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Elford H. L. Effect of hydroxyurea on ribonucleotide reductase. Biochem Biophys Res Commun. 1968 Oct 10;33(1):129–135. doi: 10.1016/0006-291x(68)90266-0. [DOI] [PubMed] [Google Scholar]
  4. Hartwell L. H., Culotti J., Pringle J. R., Reid B. J. Genetic control of the cell division cycle in yeast. Science. 1974 Jan 11;183(4120):46–51. doi: 10.1126/science.183.4120.46. [DOI] [PubMed] [Google Scholar]
  5. Hartwell L. H., Culotti J., Reid B. Genetic control of the cell-division cycle in yeast. I. Detection of mutants. Proc Natl Acad Sci U S A. 1970 Jun;66(2):352–359. doi: 10.1073/pnas.66.2.352. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hartwell L. H. Genetic control of the cell division cycle in yeast. II. Genes controlling DNA replication and its initiation. J Mol Biol. 1971 Jul 14;59(1):183–194. doi: 10.1016/0022-2836(71)90420-7. [DOI] [PubMed] [Google Scholar]
  7. Hartwell L. H., Mortimer R. K., Culotti J., Culotti M. Genetic Control of the Cell Division Cycle in Yeast: V. Genetic Analysis of cdc Mutants. Genetics. 1973 Jun;74(2):267–286. doi: 10.1093/genetics/74.2.267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hartwell L. H. Saccharomyces cerevisiae cell cycle. Bacteriol Rev. 1974 Jun;38(2):164–198. doi: 10.1128/br.38.2.164-198.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hartwell L. H. Sequential function of gene products relative to DNA synthesis in the yeast cell cycle. J Mol Biol. 1976 Jul 15;104(4):803–817. doi: 10.1016/0022-2836(76)90183-2. [DOI] [PubMed] [Google Scholar]
  10. Hutter K. J., Eipel H. E. Microbial determinations by flow cytometry. J Gen Microbiol. 1979 Aug;113(2):369–375. doi: 10.1099/00221287-113-2-369. [DOI] [PubMed] [Google Scholar]
  11. Johnston G. C., Pringle J. R., Hartwell L. H. Coordination of growth with cell division in the yeast Saccharomyces cerevisiae. Exp Cell Res. 1977 Mar 1;105(1):79–98. doi: 10.1016/0014-4827(77)90154-9. [DOI] [PubMed] [Google Scholar]
  12. Kessel M., Rosenberger R. F. Regulation and timing of deoxyribonucleic acid synthesis in hyphae of Aspergillus nidulans. J Bacteriol. 1968 Jun;95(6):2275–2281. doi: 10.1128/jb.95.6.2275-2281.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Knott G. D. Mlab--a mathematical modeling tool. Comput Programs Biomed. 1979 Dec;10(3):271–280. doi: 10.1016/0010-468x(79)90075-8. [DOI] [PubMed] [Google Scholar]
  14. Mitchison J. M., Creanor J. Induction synchrony in the fission yeast. Schizosaccharomyces pombe. Exp Cell Res. 1971 Aug;67(2):368–374. doi: 10.1016/0014-4827(71)90421-6. [DOI] [PubMed] [Google Scholar]
  15. Oujezdsky K. B., Grove S. N., Szaniszlo P. J. Morphologica and structural changes during the yeast-to mold conversion of Phialophora dermatitidis. J Bacteriol. 1973 Jan;113(1):468–477. doi: 10.1128/jb.113.1.468-477.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. PUCK T. T., STEFFEN J. LIFE CYCLE ANALYSIS OF MAMMALIAN CELLS. I. A METHOD FOR LOCALIZING METABOLIC EVENTS WITHIN THE LIFE CYCLE, AND ITS APPLICATION TO THE ACTION OF COLCEMIDE AND SUBLETHAL DOSES OF X-IRRADIATION. Biophys J. 1963 Sep;3:379–397. doi: 10.1016/s0006-3495(63)86828-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. ROSENKRANZ H. S., LEVY J. A. HYDROXYUREA: A SPECIFIC INHIBITOR OF DEOXYRIBONUCLEIC ACID SYNTHESIS. Biochim Biophys Acta. 1965 Jan 11;95:181–183. doi: 10.1016/0005-2787(65)90225-x. [DOI] [PubMed] [Google Scholar]
  18. Roberts R. L., Lo R. J., Szaniszlo P. J. Induction of synchronous growth in the yeast phase of Wangiella dermatitidis. J Bacteriol. 1980 Feb;141(2):981–984. doi: 10.1128/jb.141.2.981-984.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Roberts R. L., Lo R. J., Szaniszlo P. J. Nuclear division in temperature-sensitive multicellular mutants of Wangiella dermatitidis. J Bacteriol. 1979 Mar;137(3):1456–1458. doi: 10.1128/jb.137.3.1456-1458.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Roberts R. L., Szaniszlo P. J. Temperature-sensitive multicellular mutants of Wangiella dermatitidis. J Bacteriol. 1978 Aug;135(2):622–632. doi: 10.1128/jb.135.2.622-632.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Serna L., Stadler D. Nuclear division cycle in germinating conidia of Neurospora crassa. J Bacteriol. 1978 Oct;136(1):341–351. doi: 10.1128/jb.136.1.341-351.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Sinclair W. K. Hydroxyurea: differential lethal effects on cultured mammalian cells during the cell cycle. Science. 1965 Dec 24;150(3704):1729–1731. doi: 10.1126/science.150.3704.1729. [DOI] [PubMed] [Google Scholar]
  23. Slater M. L. Effect of reversible inhibition of deoxyribonucleic acid synthesis on the yeast cell cycle. J Bacteriol. 1973 Jan;113(1):263–270. doi: 10.1128/jb.113.1.263-270.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Slater M. L. Rapid nuclear staining method for Saccharomyces cerevisiae. J Bacteriol. 1976 Jun;126(3):1339–1341. doi: 10.1128/jb.126.3.1339-1341.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Slater M. L., Sharrow S. O., Gart J. J. Cell cycle of Saccharomycescerevisiae in populations growing at different rates. Proc Natl Acad Sci U S A. 1977 Sep;74(9):3850–3854. doi: 10.1073/pnas.74.9.3850. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Szaniszlo P. J., Hsieh P. H., Marlowe J. D. Induction and ultrastructure of the multicellular (sclerotic) morphology in Phialophora dermatitidis. Mycologia. 1976 Jan-Feb;68(1):117–130. [PubMed] [Google Scholar]
  27. Timson J. Hydroxyurea. Mutat Res. 1975;32(2):115–132. doi: 10.1016/0165-1110(75)90002-0. [DOI] [PubMed] [Google Scholar]
  28. Van Dilla M. A., Trujillo T. T., Mullaney P. F., Coulter J. R. Cell microfluorometry: a method for rapid fluorescence measurement. Science. 1969 Mar 14;163(3872):1213–1214. doi: 10.1126/science.163.3872.1213. [DOI] [PubMed] [Google Scholar]
  29. Williamson D. H. The timing of deoxyribonucleic acid synthesis in the cell cycle of Saccharomyces cerevisiae. J Cell Biol. 1965 Jun;25(3):517–528. doi: 10.1083/jcb.25.3.517. [DOI] [PMC free article] [PubMed] [Google Scholar]

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