The Association of Magnetic Resonance Imaging Measures With Cognitive Function in a Biracial Population Sample

Abstract

Background

White matter hyperintensity volume (WMHV), cerebral infarcts, and total brain volume (TBV) are associated with cognitive function, but few studies have examined these associations in the general population or whether they differ by race.

Objective

To examine the association of WMHV, cerebral infarcts, and TBV with global cognition and cognition in 5 separate domains in a biracial population sample.

Setting

A biracial community population of Chicago, Illinois.

Design

Cross-sectional population study.

Participants

The study population comprised 575 participants from the Chicago Health and Aging Project (CHAP).

Main Outcome Measures

Volumetric magnetic resonance imaging (MRI) measures of WMHV, TBV, and cerebral infarcts and detailed neuropsychological testing assessments of global cognition and 5 cognitive domains.

Results

Overall and among those without dementia, cognition was inversely associated with WMHV and number of infarcts but was positively associated with TBV. When all 3 measures were simultaneously added to the model, the association of global cognition with WMHV and TBV remained significant and unchanged but was no longer significant with infarcts. Among subjects without dementia, all 3 MRI measures were associated with performance in multiple cognitive domains, specifically perceptual speed. However, among subjects with dementia, only TBV was associated with cognition and performance in multiple cognitive systems. Race did not significantly modify any of these associations.

Conclusions

In this biracial general population sample, the associations of MRI measures with cognition differed according to clinical status of subjects (stronger among subjects without dementia) and were not modified by race. These associations did not affect all cognitive domains equally but were more consistent with impairments in perceptual speed.

WHITE MATTER HYPERintensities (WMHs), cerebral infarcts, and total brain volume (TBV) have been associated with cognitive function,^1–7 cognitive decline,^8–15 and dementia.^2,16,17 Each reportedly affects certain cognitive domains more than others.^18–25 Few studies have systematically examined these associations among subjects from the general population with adequate minority representation.^4,5,20 We used data from 575 older individuals from a biracial (white and African American) population in the Chicago Health and Aging Project (CHAP), an ongoing epidemiological study of chronic diseases in elderly individuals, to examine the association of each magnetic resonance imaging (MRI) measure with cognition globally and in 5 cognitive domains and whether these associations were modified by race or varied with clinical diagnosis.

METHODS

STUDY POPULATION

CHAP is a longitudinal population study of common chronic health problems among African American and white older adults. Its design and population characteristics have been previously reported.^26,27 Each CHAP data collection cycle has (1) an in-home population interview, with brief tests of physical and cognitive function, and (2) clinical evaluation of a stratified random sample (approximately one-sixth) of subjects that includes detailed neuropsychological testing, a neurological examination, medical history, laboratory testing, and expert clinical assessment for dementia. Clinical evaluations usually take place in subjects' homes, conducted by a team of examiners led by a senior neurologist (N.T.A.). Structured neurological examinations and medical histories were performed by specially trained nurse clinicians. A senior neuropsychologist (R.S.W.), blinded to age, sex, race, and clinical data other than the subjects' educational level, occupation, and information about sensory or motor deficits, reviewed the results of 17 cognitive performance tests and summarized impairment in each of 5 areas (orientation, attention, memory, language, and perception). Diagnosis of dementia required the study neurologist's or geriatrician's assessment of loss of cognitive function and impairment in 2 or more areas on cognitive performance testing. The diagnosis of Alzheimer disease (AD) used the criteria of the National Institute of Neurological and Communicative Disorders and Stroke–Alzheimer Disease and Related Disorders Association,²⁸ except that subjects who met these criteria and had another condition impairing cognition were retained. Vascular dementia diagnosis followed the National Institute of Neurological Association–Association Internationale pour la Recherche et l'Enseignement en Neurosciences criteria.²⁹

Of 1260 persons who completed the clinical evaluation, 663 participated MRI evaluation. Those who did not (n=597) were older (mean [SD] age, 81.5 [6.5] vs 80.1 [5.9] years; t₁₂₁₁=4.1; P>.001); less educated (mean [SD] years of education, 12.4 [3.5] vs 12.9 [3.7]; t₁₂₅₈=−2.3; P=.02); and more likely to be women (396 of 775 women vs 201 of 485 men; ${\chi }_1^2=11.15$; P=.008). No differences regarding participation were noted for race (P=.74).

The study sample comprised 575 persons for whom complete neuropsychological and MRI data were available for analyses. The 2 groups of persons with and without complete cognitive and/or neuroimaging data did not differ in clinical characteristics (age, education, sex, or race). Those included in these analyses had a mean (SD) age of 79.8 (5.9) years; completed a mean (SD) of 13.0 (3.7) years of education; and 57.0% were women (328 of 575) and 58.3%, African American (335 of 575).

There were 81 dementia cases: 6 non-AD dementia cases (7.4%) (2 vascular dementia, 1 Parkinson disease, and 3 of unknown subtype) and 75 AD dementia cases (92.6%) (61 AD and 14 AD and another condition [9 with a diagnosis of clinical stroke, 4 with depression, and 1 with Parkinson disease]). Because results from analyses performed in the AD dementia group and the full dementia group were comparable (there were too few cases of non-AD dementia to perform analyses), all analyses in this article were performed with the full dementia group. Signed informed consent was obtained from each subject, and the institutional review board of Rush University Medical Center, Chicago, Illinois. approved the study.

COGNITIVE ASSESSMENT

The 17 cognitive function tests used in analyses (Table 1) assessed cognitive abilities that may be differentially affected by aging and AD, including 7 episodic memory measures: Consortium to Establish a Registry for Alzheimer's Disease (CERAD) Word List Memory, recall and recognition,³⁰ and immediate and delayed recall of Story A from the Logical Memory subtest of the Wechsler Memory Scale–Revised³¹ and the East Boston Story³²; 3 semantic memory measures (a 15-item version of the Boston Naming Test,³³ verbal fluency, and a short form of the National Adult Reading Tests)³⁴; 3 working memory measures (digit span forward, digit span backward, and digit ordering)³⁵; 2 perceptual speed measures (an oral version of the Symbol Digit Modalities Test³⁶ and Number Comparison Test³⁷); and 2 visuo-spatial ability measures (a short form of the Judgment of Line Orientation Test³⁸ and Standard Progressive Matrices³⁹).

Table 1.

Psychometric Characteristics of Cognitive Tests in a Stratified Random Sample of the Population^a

Cognitive Domain, Test	Mean (SD) (Range)
Episodic memory
Word list memory	12.45 (6.01) (0–27)
Word list recall	3.11 (2.79) (0–10)
Word list recognition	7.71 (3.10) (0–10)
East Boston immediate	7.07 (3.53) (0–12)
East Boston delayed	6.02 (3.40) (0–12)
Logical memory: Ia	6.65 (4.94) (0–21)
Logical memory: IIb	4.99 (4.68) (0–20)
Semantic memory
Boston naming	11.03 (3.57) (0–15)
Category fluency	22.90 (11.42) (0–58)
Reading test	7.23 (4.95) (0–15)
Working memory
Digits span forward	6.44 (2.51) (0–12)
Digits span backward	4.01 (2.45) (0–12)
Digit ordering	5.03 (2.68) (0–12)
Perceptual speed
Symbol digit	21.52 (15.69) (0–59)
Number comparison	15.54 (9.39) (0–42)
Visuospatial ability
Judgment of line orientation	6.64 (4.62) (0–15)
Standard progressive matrices	7.63 (3.10) (0–12)

^aUsed in computing zscores for cognitive measures.

In a previous study of 1399 persons without dementia in this cohort,⁴⁰ we performed a factor analysis of the 17 tests with varimax rotation. The hypothesized factor analytic grouping of these tests showed good agreement with the empirical results of the factor analysis: the Rand statistic,⁴¹ a measure of goodness of fit ranging from −1 to 1, was 0.62 (P>.001). To minimize floor and ceiling effects, we constructed summary measures of global cognition and each of 5 cognitive domains into which the tests could hypothetically be grouped, rather than using individual tests scores in analyses. Each summary measure was constructed by converting raw scores from individual tests to z scores using the mean and standard deviation from the baseline clinical evaluation of all participants in the CHAP study and averaging the z scores. Valid summary measures required valid scores on at least half of the component tests. The global cognitive summary averaged the z scores of all 17 tests. Several studies characterizing cognitive function using this approach in this and other cohorts have been previously reported.^42–45

MRI EVALUATION

Subjects were imaged on a General Electric 1.5-T scanner (Excite platform, version 11; General Electric Healthcare, Milwaukee, Wisconsin), and the following imaging sequences were obtained: (1) fluid-attenuated inversion recovery (FLAIR): repetition time (TR)=11 000 milliseconds (ms), echo time (TE)=144 ms, inversion time (TI)=2250 ms, 22-cm field of view (FOV), 3-mm slice thickness, 192×256 acquisition matrix; (2) SPGR: TE minimum, 20° flip angle, 24-cm FOV, and 1.5-mm slice thickness, with 256×256 acquisition matrix; and (3) double-spin echo: TR=2100 ms, TE=30/92 ms, 22-cm FOV, and 4-mm slice thickness, with 256/192 acquisition matrix. Images were oriented parallel to a hypothetical line connecting the anterior commissure and posterior commissure. After acquiring the MRI scans, the digital information was transferred to a central laboratory directed by one of us (C.D.) for processing and analysis. Imaging analyses were performed blind to personal identifying information and used QUANTA 6.2, operating on a Sun Microsystems (Santa Clara, California) Ultra 5 workstation.

White matter hyperintensity segmentation was performed using a 2-step process as reported previously.^46–48 Briefly, nonbrain elements were manually removed from the image, and the resulting measure of the cranial vault was defined as the total cranial volume to correct for head size differences among subjects. The first step in image segmentation required identifying brain matter. Image intensity nonuniformities were then removed, and the corrected image was modeled as a mixture of 2 gaussian probability functions, with the segmentation threshold determined at the minimum probability between the two.^46,49 Once brain matter segmentation was achieved, a single gaussian distribution was fitted to image data, and a segmentation threshold for WMH volume (WMHV) was determined a priori as 3.5 SDs in pixel intensity above the mean of the fitted distribution of brain parenchyma.⁵⁰ Morphometric erosion of 2 exterior image pixels was also applied to the brain matter image before modeling to remove the effect of partial volume cerebrospinal fluid pixels on WMH determination. White matter hyperintensity volume was calculated as a proportion of total cranial volume (to account for head size variation among participants) and log transformed (natural log) to achieve a normal distribution (skew, −0.21). Total brain volume was computed as the ratio of total brain parenchymal volume to total cranial volume and had approximately a normal distribution (skew, −0.10). The presence or absence of cerebral infarcts was determined manually by the operator based on the lesion's size and imaging characteristics.⁵¹ The image analysis system allowed for superimposition of the FLAIR image, proton-density image, and T2-weighted image at 3-times magnified view to assist in interpreting lesion characteristics. Signal void, seen on T2-weighted images, was interpreted to indicate a vessel. Lesions 3 mm or larger were considered brain infarcts. Other necessary imaging characteristics included (1) cerebrospinal fluid density on the subtraction image and (2) whether the infarct was in the basal ganglia, distinct separation from the circle of Willis vessels and perivascular spaces. Interrater reliability for the MRI measures have been previously published,^52–54 and intrarater and interrater reliabilities for this study were consistently above 0.90. Because the number of cerebral infarcts had a skewed distribution, for analyses we used as a reference group those without infarcts. We then compared that group with the group with 1 infarct and the group with more than one,⁵⁵ as well as with those with only small infarcts (<1 cm), only large infarcts (≥1 cm), and both small and large infarcts.

STATISTICAL ANALYSIS

Using linear regression analyses, we first assessed the association of MHV and our measure of TBV with Pearson correlation coefficients and the association of WMHV and TBV with having cerebral infarcts (1 and >1) To test for differences in demographics and neuroimaging measures among African Americans and whites and those with and without dementia, we used t tests or analysis of variance for continuous variables and χ² tests for categorical data. P<.05 was considered statistically significant unless otherwise specified.

We next assessed the associations of WMHV, TBV, and cerebral infarcts with cognition globally and within each of the 5 cognitive systems, using linear regression analyses, considering each MRI measure individually and the 3 jointly. All core models controlled for age, sex, race, education, and time elapsed between the clinical evaluation and brain MRI. Because there were 6 outcomes, we used a P value of .008 (.05/6) for a 2-sided test as a cutoff score of significance for these analyses. In additional analyses, we evaluated whether these associations varied according to the presence of dementia or by race/ethnicity, using interaction terms. Model assumptions about linearity, normality, independence, and homoscedasticity of errors were assessed graphically and analytically and were adequately met. Analyses were performed using SAS/STAT software version 8.⁵⁶

RESULTS

Increased WMHV was associated with decreased TBV (r₅₇₃=−0.22; P<.001) and having single (coefficient estimate [SE], 59.3 [10.4]; P<.001) or multiple (coefficient estimate [SE], 101.5 [14.7]; P<.001) infarcts. Decreased TBV was associated with having multiple infarcts (coefficient estimate [SE], −1.84 [0.58]; P=.002) and showed a trend toward association with having a single infarct (coefficient estimate [SE], −0.72 [0.41]; P=.08). Increased age was associated with higher WMHV (r₅₇₃=0.31; P<.001), lower TBV (r₅₇₃=−0.41; P<.001), and having 1 infarct (F_2,577=3.43; P=.03). Lower levels of education were associated with lower TBV (r₅₇₃=0.09; P=.04) and having 1 infarct (F_2,577=4.4; P=.01). Male sex was associated with lower TBV (t₅₇₃=5.46; P<.001).

Demographic and neuroimaging characteristics of the sample are given in Table 2, stratified by race. African Americans had fewer years of education and lower cognitive test scores. There was a trend toward a greater proportion of whites with multiple infarcts, but no significant differences were noted in any neuroimaging measures across the racial groups.

Table 2.

Demographic, Neuropsychological, and Magnetic Resonance Imaging Characteristics of 575 Participants Stratified by Race/Ethnicity

Variable	All Subjects (N = 575)	African American (n = 335)	White (n = 240)	P Value for Differencea
Age, mean (SD), y	79.8 (5.9)	79.5 (6.0)	80.1 (5.8)	.22
Education, mean (SD), y	13.0 (3.7)	11.6 (3.3)	14.9 (3.4)	<.001
MMSE score (maximum = 30), mean (SD)	26.2 (3.8)	25.4 (4.0)	27.3 (3.4)	<.001
Global cognitive score, mean (SD)	0.46 (0.6)	0.74 (0.5)	0.74 (0.5)	<.001
WMHV, mean (SD)	−5.13 (1.09)	−5.16 (1.10)	−5.09 (1.07)	.49
TBV, mean (SD)	74.29 (5.85)	74.94 (4.72)	74.00 (4.40)	.20
Male sex, No. (%)	247 (43.0)	146 (43.6)	101 (42.1)	.62
1 Infarct, No. (%)	108 (18.8)	62 (18.5)	45 (18.8)	NA
>1 Infarct, No. (%)	47 (8.2)	21 (6.3)	27 (11.3)	.09b

Abbreviations: MMSE, Mini-Mental State Examination; NA, not applicable; TBV, total brain volume (TBV = total brain parenchymal volume/total cranial volume); WMHV, white matter hyperintensity volume (WMHV = natural log [white matter hyperintensity volume/total cranial volume]).

^aPvalue based on t test for continuous variables and χ² test for categorical variables.

^bBased on group by number of infarcts (0, 1, or >1); χ² association test.

MRI MEASURES AND GLOBAL COGNITIVE FUNCTION

Among all subjects, higher WMHV and having more than 1 infarct were associated with lower global cognitive function, and higher TBV was associated with better cognitive function (Table 3). When all 3 MRI measures were considered simultaneously in a model, the associations of WMHV (adjusted estimate [SE], −0.109 [0.018]; P<.001) and TBV (adjusted estimate [SE], 0.031 [0.005]; P<.001) with global cognition were not substantially changed, but infarcts were no longer associated with global cognition (adjusted estimate [SE], −0.050 [0.066]; P=.45). For all 3 MRI measures, the association with global cognitive function was not modified by race/ethnicity (Table 4). The Figure shows predicted cognition vs each MRI measure for African Americans and whites. Although the intercepts of the 2 lines differed significantly, the relationship between MRI measure and cognition was similar in both groups.

Table 3.

Association of Magnetic Resonance Imaging Measures to Global Cognitive Function and 5 Different Cognitive Domains^a

	Total Sample (N = 575)		No Dementia (n = 494)		Dementia (n = 81)
Cognitive Function, Model Term	Estimate (SE)	P Value	Estimate (SE)	P Value	Estimate (SE)	P Value
Global cognitive function
WMHV	−0.108 (0.018)	<.001	−0.058 (0.015)	<.001	−0.035 (0.049)	.48
1 Infarct	0.043 (0.047)	.37	0.016 (0.039)	.68	0.090 (0.117)	.43
>1 Infarct	−0.215 (0.067)	.002	−0.154 (0.059)	.009	−0.157 (0.138)	.26
TBV	0.032 (0.005)	<.001	0.017 (0.004)	<.001	0.035 (0.009)	<.001
Episodic memory
WMHV	−0.125 (0.022)	<.001	−0.080 (0.020)	<.001	0.033 (0.064)	.61
1 Infarct	0.104 (0.059)	.08	0.045 (0.051)	.38	0.282 (0.152)	.07
>1 Infarct	−0.238 (0.085)	.005	−0.159 (0.077)	.04	−0.249 (0.174)	.16
TBV	0.029 (0.006)	<.001	0.010 (0.006)	.07	0.034 (0.012)	.007
Semantic memory
WMHV	−0.099 (0.023)	<.001	−0.047 (0.021)	.03	−0.115 (0.080)	.16
1 Infarct	0.040 (0.059)	.054	0.019 (0.054)	.718	−0.038 (0.187)	.84
>1 Infarct	−0.179 (0.084)	.04	−0.155 (0.081)	.06	−0.126 (0.215)	.56
TBV	0.031 (0.006)	<.001	0.017 (0.006)	.004	0.047 (0.014)	.001
Working memory
WMHV	−0.022 (0.024)	.006	−0.022 (0.024)	.37	−0.036 (0.075)	.63
1 Infarct	−0.019 (0.063)	.76	−0.040 (0.063)	.53	−0.031 (0.182)	.87
>1 Infarct	−0.051 (0.216)	.03	−0.170 (0.096)	.08	−0.051 (0.216)	.81
TBV	0.029 (0.006)	<.001	0.016 (0.070)	.02	0.035 (0.015)	.02
Perceptual speed
WMHV	−0.156 (0.028)	<.001	−0.103 (0.028)	<.003	−0.011 (0.071)	.88
1 Infarct	−0.205 (0.172)	.57	0.033 (0.074)	.65	0.205 (0.172)	.24
>1 Infarct	−0.375 (0.106)	0.1	−0.325 (0.116)	.004	−0.102 (0.199)	.61
TBV	0.053 (0.007)	<.001	0.041 (0.008)	<.001	0.033 (0.014)	.02
Visuospatial ability
WMHV	−0.110 (0.024)	<.001	−0.066 (0.024)	.006	−0.098 (0.077)	.21
1 Infarct	−0.051 (0.063)	.43	−0.069 (0.626)	.27	−0.060 (0.195)	.76
>1 Infarct	−0.123 (0.091)	.18	−0.088 (0.095)	.36	0.058 (0.225)	.80
TBV	0.033 (0.006)	<.001	0.022 (0.007)	<.001	0.032 (0.015)	.04

Abbreviations: TBV, total brain volume (TBV = total brain parenchymal volume/total cranial volume); WMHV, white matter hyperintensity volume (WMHV = natural log [white matter hyperintensity volume/total cranial volume]).

^aEstimated from separate linear regression models that controlled for age, sex, education, race, and time elapsed between clinical evaluation and magnetic resonance imaging. The α level was set at .008 to account for the 6 outcome measures (ie, .05/6 = .008).

Table 4.

Linear Regression Models Examining the Associations of MRI Measures With Global Cognitive Function and Potential Modification by Race/Ethnicity^a

	Model 1		Model 2
Model Terms	Estimate (SE)	P Value	Estimate (SE)	P Value
Race	−0.295 (0.040)	<.001	−0.045 (0.174)	.80
WMHV	−0.108 (0.018)	<.001	−0.137 (0.027)	<.001
WMHV × race	NA	NA	0.049 (0.033)	.14
Race	−0.302 (0.040)	<.001	0.071 (0.589)	.90
TBV	0.032 (0.005)	<.001	0.035 (0.007)	<.001
TBV × race	NA	NA	−0.005 (0.008)	.53
Race	−0.300 (0.042)	<.001	−0.274 (0.047)	<.001
1 Infarct	−0.215 (0.068)	.002	−0.042 (0.095)	.66
>1 Infarct	NA	NA	−0.216 (0.136)	.11

Abbreviations: MRI, magnetic resonance imaging; NA, not applicable; TBV, total brain volume (TBV = total brain parenchymal volume/total cranial volume); WMHV, white matter hyperintensity volume (WMHV = natural log [white matter hyperintensity volume/total cranial volume]).

^aFor each MRI measure, model 2 adds an interaction term to assess whether race modifies the association of each measure with level of cognitive function. All models controlled for age, sex, race, education, and time elapsed between the clinical evaluation and MRI.

Figure.

Predicted relation between white matter hyperintensity volume and global cognitive function (A) and total brain volume and global cognitive function (B) for African Americans (solid line) and whites (dashed line). White matter hyperintensity volume=natural log (white matter hyperintensity volume/total cranial volume). Total brain volume=total brain parenchymal volume/total cranial volume. Dotted lines are 95% confidence intervals for the regression line.

In separate analyses of those with (n=81) and without (n=494) dementia, subjects with dementia were older, had fewer years of education, were more likely to be African American, and had lower WMHV and TBV (Table 5). Overall, the associations of MRI measures with global cognition were stronger among subjects without dementia (Table 3). Among persons without dementia, those with higher WMHV had a strong inverse association with global cognition, but among persons with dementia, the association was not significant. Having multiple infarcts also had an inverse association with global cognition among subjects without dementia but not those with dementia. Because infarct size is thought to be associated with poor cognitive function and dementia, we conducted additional analyses in our dementia and nondementia groups with a measure of infarct size. There was no association of infarct size with global cognition in both the nondementia and dementia groups (results not shown). Total brain volume was positively associated with better cognition among those both with dementia and without.

Table 5.

Sample Characteristics for Persons According to the Presence or Absence of Dementia

Variable	No Dementia (n = 494)	Dementia (n = 81)	P Value
Age, mean (SD), y	79.5 (5.9)	81.8 (5.4)	<.001
Education, mean (SD), y	13.1 (3.6)	11.8 (4.3)	.006
WMHV, mean (SD)	−5.22 (1.08)	−4.50 (0.93)	<.001
TBV, mean (SD)	74.65 (4.4)	71.97 (5.3)	<.001
Male sex, No. (%)	213 (43.1)	34 (42.0)	.98
African American, No. (%)	280 (56.7)	55 (67.9)	.02
1 Infarct, No. (%)	93 (18.8)	14 (17.3)	NA
>1 Infarct, No. (%)	37 (7.5)	11 (13.5)	.18a

Abbreviations: NA, not applicable; TBV, total brain volume (TBV = total brain parenchymal volume/total cranial volume); WMHV, white matter hyperintensity volume (WMHV = natural log [white matter hyperintensity volume/total cranial volume]).

^aBased on group by number of infarcts (0, 1, or >1); χ² association test.

Among subjects without dementia, if all 3 MRI measures were considered together in a single model, the association of cerebral infarcts with cognitive function was no longer significant, whereas the association of WMHV and TBV with cognition remained significant and essentially unchanged (Table 6).

Table 6.

Association of WMHV, TBV, and Multiple Infarcts With Global Cognitive Function^a

Model, Predictors	Estimate (SE)	P Value
Model 1
WMHV	−0.058 (0.015)	<.001
Model 2
WMHV	−0.056 (0.015)	<.001
TBV	0.016 (0.004)	<.001
Model 3
WMHV	−0.055 (0.016)	<.001
TBV	0.017 (0.004)	<.001
>1 Infarct	−0.085 (0.060)	.16

Abbreviations: TBV, total brain volume (TBV = total brain parenchymal volume/total cranial volume); WMHV, white matter hyperintensity volume (WMHV = natural log [white matter hyperintensity volume/total cranial volume]).

^aEstimated from separate linear regression models that controlled for age, sex, education, race, and time elapsed between clinical evaluation and magnetic resonance imaging.

MRI MEASURES AND AREAS OF COGNITIVE FUNCTION

In persons without dementia, having multiple cerebral infarcts was inversely associated with perceptual speed but not with performance in any other cognitive domain (Table 3). In separate analyses, we added a term for our measures of infarct size. Overall, there was no association of infarct size with any of the 5 cognitive domains in both the nondementia and dementia groups (results not shown). The association of WMHV with cognitive domain performance appeared stronger among persons without dementia. There were significant associations noted with performance in 3 of the 5 domains in this subgroup but with none of the 5 domains among persons with dementia.

COMMENT

In this cross-sectional study of more than 575 elderly individuals from a biracial population sample, we found that when considered singly, WMHV, TBV, and having multiple cerebral infarcts were associated with global cognitive function. Overall and among those without dementia, cognition was inversely associated with WMHV and number of infarcts but was positively associated with TBV. When all 3 measures were simultaneously added to the model, the association of global cognition with WMHV and TBV remained significant and unchanged but was no longer significant with infarcts. There was a non-significant trend for having multiple infarcts in a higher proportion of white subjects, but WMHV was similar in both groups. While African Americans performed more poorly on all cognitive tests, the associations of all 3 MRI measures with cognitive function were not modified by race. Finally, the inverse associations of both WMHV and multiple infarcts with global cognition were much stronger among subjects without dementia, and the strength of the associations with cognition varied across specific cognitive domains.

Few studies have examined the associations of multiple MRI measures with cognitive function in general population samples of older persons. Results of the 3 studies published in the last decade suggest that WMHV, TBV, and infarcts each are associated with cognitive function, but when considered simultaneously, such associations are mixed.^2,17 One study found that the association of cognition with WMHV was independent of its association with infarcts but not with TBV¹⁷; another found that the association of global cognition with infarcts was independent of both its associations with TBV and WMHV.² Neither of these studies used quantitative measures of WMHV or TBV; thus, the extent to which the differences in findings are attributable to methodological differences is uncertain. The results presented from this study, that the strength of the association of WMHV with cognitive function was unchanged in the presence of cerebral infarcts, are in keeping with those of others⁵ and suggest that WMHV may better reflect overall vascular brain injury and therefore have a stronger association with cognitive function.

The association of WMHV with global cognition and with performance in specific cognitive domains (episodic memory, perceptual speed, and visuospatial ability) was much stronger among subjects without dementia. A possible partial explanation is the disparity in these groups' sizes (81 subjects with dementia and 494 without). Another possibility, however, is that the association of WMHV with cognition may be more important at earlier stages (ie, before subjects meet criteria for clinical diagnosis of dementia). Recent data suggest that higher WMHV is associated with increased risk of progression from no cognitive impairment to mild cognitive impairment but not from mild cognitive impairment to dementia.^13,57 The underlying mechanism may relate to vascular brain injury, in view of the association of WMHV with vascular disease^10,23,48,51 and the reported association of vascular disease and vascular risk factors with impaired cognition.²⁵ White matter hyperintensities are thought to reflect diffuse ischemic changes in the brain, which disrupt both the frontal subcortical circuits that subserve predominantly executive cognitive abilities^58–60 and the connections between the frontal lobes and other cortical regions. White matter hyperintensity distribution over many anatomical areas of the brain could explain the correspondingly wide effects on the cognitive systems subserved by these areas, with maximal effects occurring before the pathological processes associated with dementia (reflected by brain volume loss) begin. Also possible is that many subjects with dementia had mixed pathological features⁶¹ including AD. This could explain the continued significant association between brain atrophy and cognition, but not WMHs or infarcts and cognition, in this subgroup, since brain atrophy is affected by both pathological processes.⁶²

Some studies suggest that WMHs and TBV are associated with function in multiple cognitive domains,^18,19,23,24 in contrast to cerebral infarcts, which affect predominantly measures of executive function.^2,12,25 Our results generally agree: the measure of executive function performance (perceptual speed) was the domain most consistently associated with each MRI measure. We found that having multiple infarcts was associated with performance in episodic memory (typically prominently affected in AD) in the entire sample, and preliminary data suggest a similar, albeit weaker, association among subjects without dementia. These results are similar to those reported from 2 recent studies in older persons without dementia.^63,64 The underlying mechanisms by which cerebral infarcts could affect cognition in elderly individuals, specifically in the domain of memory, are unclear; one possibility is that infarcts have an additive effect on subclinical preexisting AD pathology that varies with the anatomical locations of the infarcts. Although a high proportion of our subjects without dementia (130 of 494) had infarcts seen on MRI, information on the location of these infarcts will be needed to address this issue further.

Few studies have examined differences in brain MRI findings between African Americans and whites.^7,62,65-67

In the present study, we examined 2 potential differences: first, differences in the amount or degree of a brain measure between these racial groups, and second, differences according to race in the association of brain MRI measures with cognition. Unlike other studies suggesting that both WMHs and cerebral infarcts^66,67 may be more prevalent among African Americans, in this sample we found a trend toward a higher proportion of whites with multiple infarcts. One possible explanation is that the overall occurrence of vascular risk factors in African Americans in our sample may have been lower than in previous studies. Further studies in this sample are planned to closely examine the association of vascular disease and risk factors and their associations with brain MRI measures.

Consistent with a previous study,⁵ the association of each MRI measure with cognitive function was similar between African Americans and whites. Despite differences in baseline cognitive test results that persisted after controlling for educational achievement, both groups showed similar associations between cognitive performance and MRI pathological measures, suggesting that race itself does not modify the association of MRI measures with cognitive function. Longitudinal assessment of cognitive function in relation to brain measures is needed to further examine these preliminary observations.

This study has important strengths and limitations. Strengths include the inclusion of a large, well-characterized, biracial cohort of older persons; the use of previously established and validated measures of cognitive function; and the use of identical neuroimaging protocols at the same MRI facility. Limitations include the cross-sectional design, losses to MRI participation, and use of rough measures of infarct status (number and size vs volume measurements). Finally, though this study examined the association of multiple MRI measures with cognitive function in persons with dementia (the majority of which were AD), the paucity of cases of mixed dementia and vascular dementia limited our ability to perform specific analyses to examine the association between multiple MRI measures and cognitive function in these dementia subtypes. It will be important to examine these associations in future studies with larger sample sizes.