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. 1980 Dec;144(3):999–1002. doi: 10.1128/jb.144.3.999-1002.1980

Mechanism of additive genetic transformation in Haemophilus influenzae.

J H Stuy
PMCID: PMC294763  PMID: 6969256

Abstract

Transforming deoxyribonucleic acid (DNA) preparations from Haemophilus influenzae Rd strains carrying a chromosomally integrated, conjugative, antibiotic resistance transfer (R) plasmid were exposed to ultraviolet radiation and then assayed for antibiotic resistance transfer on sensitive wild-type Rd competent suspensions and on similar suspensions of a uvr-1 mutant unable to excise pyrimidine dimers. No host cell reactivation of resistance transfer (DNA repair) was observed. Parallel experiments with ethanol-precipitated, heated, free R plasmid DNA preparations gave much higher survival when assayed on the wild-type strain compared to the survival on the uvr-1 strain. These observations indicate that additive genetic transformation (in this case, the addition of the integrated R plasmid to the recipient genome) involves single-strand insertion.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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