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. 1980 Dec;144(3):1176–1178. doi: 10.1128/jb.144.3.1176-1178.1980

Simplified fluctuation test to distinguish liquid holding recovery from cell multiplication in ultraviolet-irradiated Escherichia coli.

B R Aragão, A C Araújo, R A Gomes
PMCID: PMC294786  PMID: 7002909

Abstract

Improved plating techniques and a fluctuation test have been used to distinguish between liquid holding recovery of ultraviolet-irradiated cells and cell multiplication in Escherichia coli. Among tested strains, only recA mutants showed true recovery.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDERSON E. H. Heat reactivation of ultraviolet-inactivated bacteria. J Bacteriol. 1951 Apr;61(4):389–394. doi: 10.1128/jb.61.4.389-394.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Aragão B. R., Gomes R. A. Liquid holding recovery in E. coli K12. I - Substances released during buffer holding. Rev Bras Pesqui Med Biol. 1979 Jun;12(2-3):117–122. [PubMed] [Google Scholar]
  3. Aragão B. R., Gomes R. A. Liquid holding recovery in E. coli K12. II. Role of cellular multiplication. Rev Bras Pesqui Med Biol. 1979 Dec;12(6):371–376. [PubMed] [Google Scholar]
  4. Ganesan A. K., Smith K. C. Dark recovery processes in Escherichia coli irradiated with ultraviolet light. I. Effect of rec mutations on liquid holding recovery. J Bacteriol. 1968 Aug;96(2):365–373. doi: 10.1128/jb.96.2.365-373.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Harm W. Dark repair of photorepairable UV lesions in Escherichia coli. Mutat Res. 1968 Jul-Aug;6(1):25–35. doi: 10.1016/0027-5107(68)90100-0. [DOI] [PubMed] [Google Scholar]
  6. Howard-Flanders P., Theriot L. Mutants of Escherichia coli K-12 defective in DNA repair and in genetic recombination. Genetics. 1966 Jun;53(6):1137–1150. doi: 10.1093/genetics/53.6.1137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kaplan R., Apirion D. The fate of ribosomes in Escherichia coli cells starved for a carbon source. J Biol Chem. 1975 Mar 10;250(5):1854–1863. [PubMed] [Google Scholar]
  8. Lederberg J. Gene Recombination and Linked Segregations in Escherichia Coli. Genetics. 1947 Sep;32(5):505–525. doi: 10.1093/genetics/32.5.505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Luria S. E., Delbrück M. Mutations of Bacteria from Virus Sensitivity to Virus Resistance. Genetics. 1943 Nov;28(6):491–511. doi: 10.1093/genetics/28.6.491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Roberts R. B., Aldous E. RECOVERY FROM ULTRAVIOLET IRRADIATION IN ESCHERICHIA COLI. J Bacteriol. 1949 Mar;57(3):363–375. doi: 10.1128/jb.57.3.363-375.1949. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Tang M. S., Patrick M. H. Repair of UV damage in Escherichia coli under non-growth conditions. Photochem Photobiol. 1977 Sep;26(3):247–255. doi: 10.1111/j.1751-1097.1977.tb07481.x. [DOI] [PubMed] [Google Scholar]
  12. Tang M. S., Patrick M. H. The role of DNA polymerase I in liquid holding recovery of UV-irradiated Escherichia coli. Photochem Photobiol. 1977 Sep;26(3):257–262. doi: 10.1111/j.1751-1097.1977.tb07482.x. [DOI] [PubMed] [Google Scholar]
  13. Tang M. S., Wang T. C., Patrick M. H. DNA turnover in buffer-held Escherichia coli and its effect on repair of UV damage. Photochem Photobiol. 1979 Mar;29(3):511–520. doi: 10.1111/j.1751-1097.1979.tb07083.x. [DOI] [PubMed] [Google Scholar]

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