Abstract
Four lysozyme-sensitive mutants were isolated after nitrosoguanidine mutagenesis of a lysozyme-insensitive strain of Staphylococcus aureus. One mutant was sufficiently effective for the isolation of macromolecules, such as plasmid deoxyribonucleic acids, from a cell after lysozyme-induced cell lysis.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Amano K., Hayashi H., Araki Y., Ito E. The action of lysozyme on peptidoglycan with N-unsubstituted glucosamine residues. Isolation of glycan fragments and their susceptibility to lysozyme. Eur J Biochem. 1977 Jun 1;76(1):299–307. doi: 10.1111/j.1432-1033.1977.tb11596.x. [DOI] [PubMed] [Google Scholar]
- BRUMFITT W., WARDLAW A. C., PARK J. T. Development of lysozyme-resistance in Micrococcus lysodiekticus and its association with an increased O-acetyl content of the cell wall. Nature. 1958 Jun 28;181(4626):1783–1784. doi: 10.1038/1811783a0. [DOI] [PubMed] [Google Scholar]
- Chatterjee A. N. Use of bacteriophage-resistant mutants to study the nature of the bacteriophage receptor site of Staphylococcus aureus. J Bacteriol. 1969 May;98(2):519–527. doi: 10.1128/jb.98.2.519-527.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Clewell D. B., Helinski D. R. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159–1166. doi: 10.1073/pnas.62.4.1159. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Guerry P., LeBlanc D. J., Falkow S. General method for the isolation of plasmid deoxyribonucleic acid. J Bacteriol. 1973 Nov;116(2):1064–1066. doi: 10.1128/jb.116.2.1064-1066.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hayashi H., Araki Y., Ito E. Occurrence of glucosamine residues with free amino groups in cell wall peptidoglycan from bacilli as a factor responsible for resistance to lysozyme. J Bacteriol. 1973 Feb;113(2):592–598. doi: 10.1128/jb.113.2.592-598.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Novick R. Plasmid-protein relaxation complexes in Staphylococcus aureus. J Bacteriol. 1976 Sep;127(3):1177–1187. doi: 10.1128/jb.127.3.1177-1187.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SCHINDLER C. A., SCHUHARDT V. T. LYSOSTAPHIN: A NEW BACTERIOLYTIC AGENT FOR THE STAPHYLOCOCCUS. Proc Natl Acad Sci U S A. 1964 Mar;51:414–421. doi: 10.1073/pnas.51.3.414. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shaw D. R., Chatterjee A. N. O-Acetyl groups as a component of the bacteriophage receptor on Staphylococcus aureus cell walls. J Bacteriol. 1971 Oct;108(1):584–585. doi: 10.1128/jb.108.1.584-585.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Strominger J. L., Ghuysen J. M. Mechanisms of enzymatic bacteriaolysis. Cell walls of bacteri are solubilized by action of either specific carbohydrases or specific peptidases. Science. 1967 Apr 14;156(3772):213–221. doi: 10.1126/science.156.3772.213. [DOI] [PubMed] [Google Scholar]