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. Author manuscript; available in PMC: 2011 Mar 30.
Published in final edited form as: Oncogene. 2010 Aug 2;29(39):5427–5436. doi: 10.1038/onc.2010.276

Figure 2.

Figure 2

P68 interacted with Snail1 promoter.

(A) & (B) Chromatin immunoprecipitations (ChIP) of the Snail1 promoter by antibody Pabp68 (A, Anti-p68) and by anti-HA antibody (B, Anti-HA) in SW620 cells with/without (p68/NT) p68 knockdown. The HA-p68s (WT or Y593F mutant) were exogenously expressed. The primer positions for Snail1 PCR were indicated by arrows. ChIP by mouse IgG and antibody against RNA polymerase II (POL II), which binds to the GAPDH promoter, were used as controls. Inputs were PCR products from DNA extracts without ChIP (use 10% of sample). The lower panel shows the quantization ChIP results by real-time PCR. The ChIP quantization is expressed as a percentage of the input by defining the input as 100. The error bars represent standard deviation of results from three independent measurements. (C) Cellular levels of E-cadherin (second panel from top) and vimentin (third panel from top) were detected by IBs of cellular extracts made from SW620 cells with/without (Snail1/NT) Snail1 siRNA knockdown and exogenous expression of HA-p68s (WT or Y593F mutant). Tyrosine phosphorylation of HA-p68s was analyzed by IB of anti-HA IPs via antibody p-Tyr-100 (fifth panel from top). IB of histone 2A (H2A) was loading control.