Figure 3.
Analysis of the hematopoietic stem cell compartment in Cdx4F/F-Cre+ mice. (A) Flow cytometric analysis of the LSK (Lin−c-Kit+Sca1+) and myeloid progenitor compartments 4–6 weeks after pIpC treatment. Left panels show 10000 cells gated on viable Lin− cells. Right panels show 10000 cells gated on viable Lin− c-Kit+Sca1− cells (B) Histogram representation of results presented in (A). Mean±SD are shown (n=4). (C) Diagram showing non-competitive and competitive bone marrow repopulation assays. (D) Contribution of donor cells (CD45.2+CD45.1−) to the hematopoiesis of lethally irradiated recipients in non-competitive and competitive transplants. The percentages of CD45.2+ and CD45.1+ cells in the peripheral blood of recipient mice were measured by flow cytometric analysis at 4, 8, 12 and 16 weeks after transplantations (n=5). Mean±SD of the percentage of CD45.2+ cells are shown.