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Effects of metal ions (Mg2+ and Mn2+), EDTA, and DTT (in the presence of 20 mM Mg2+) on the fucosyltransferase activity of MBP–HhFT1–His6 when LacNAcβPro2AA (white columns) or Neu5Acα2-3LacNAcβPro2AA (black columns) was used as an acceptor substrate.
