Fig. 2.

A nondenaturing, discontinuous polyacrylamide gel shows that lysates of Giardia contain two proteins with PGM activities, one of which also has PMM activity. Giardia lysates were tested for PGM activity (lane 1) and PMM activity (lane 2). The single arrow identifies a fast migrating Giardia protein, which only has PGM activity. The double arrow identifies a slower migrating Giardia protein, which has both PGM and PMM activities. The dashed arrow is the interface between stacking and running gels. Lysates of wild-type Saccharomyces (Sc WT in lane 3) have PGM activity, which is absent from lysates of the Saccharomyces pgm1Δ/pgm2Δ double deletion strain (lane 4)